| A型口蹄疫病毒（FMDV）VP1基因遗传进化分析 |
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| 引用本文： | 杨江,范辰韵,高文倩,罗晓林,安添午,庞倩,许路来,徐岳松,徐鹏卫,杨鑫.A型口蹄疫病毒（FMDV）VP1基因遗传进化分析[J].四川大学学报(自然科学版),2016,53(2):430-436. |
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| 作者姓名： | 杨江 范辰韵 高文倩 罗晓林 安添午 庞倩 许路来 徐岳松 徐鹏卫 杨鑫 |
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| 作者单位： | 四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室,
“985工程”西南资源环境与灾害防治科技创新平台;四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室,
“985工程”西南资源环境与灾害防治科技创新平台;四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室,
“985工程”西南资源环境与灾害防治科技创新平台;四川省草原科学研究院;四川省草原科学研究院;四川省草原科学研究院;四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室,
“985工程”西南资源环境与灾害防治科技创新平台;四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室,
“985工程”西南资源环境与灾害防治科技创新平台;四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室,
“985工程”西南资源环境与灾害防治科技创新平台;四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室,
“985工程”西南资源环境与灾害防治科技创新平台 |
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| 基金项目： | 四川省科技支撑计划(2012NZ0047); 四川省科技基础条件平台项目(14010136); 国家自然科学基金项目(J1103518) |
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| 摘 要： | 对疑似感染FMDV的样本进行了FMDV VP1基因RT-PCR鉴定,测定了其中1个阳性样本序列(命名为HY).采用生物信息学软件比较了HY与参考序列的同源性,并构建VP1基因的分子系统树.结果表明:HY属A型FMDV亚洲拓扑型东南亚-97谱系,与我国近年来报道的A/GDMM/CHA/2013的序列相似性为99.1%,与AF/72疫苗株的相似性仅为79.0%.提示我国近年来报道的A型毒株与AF/72疫苗株的序列差异较大,应及时更换A型口蹄疫疫苗毒株.
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| 关 键 词： | 口蹄疫病毒 VP1 A型 进化树分析 |
| 收稿时间： | 2014/11/27 0:00:00 |
| 修稿时间： | 2015/1/13 0:00:00 |
The Phylogenetic Analysis of Foot-and-mouth Disease Virus (FMDV) Serotype A Based on VP1 Gene |
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| YANG Jiang,FAN Chen-Yun,GAO Wen-Qian,LUO Xiao-Lin,AN Tian-Wu,PANG Qian,XU Lu-Lai,XU Yue-Song,XU Peng-Wei and YANG Xin.The Phylogenetic Analysis of Foot-and-mouth Disease Virus (FMDV) Serotype A Based on VP1 Gene[J].Journal of Sichuan University (Natural Science Edition),2016,53(2):430-436. |
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| Authors: | YANG Jiang FAN Chen-Yun GAO Wen-Qian LUO Xiao-Lin AN Tian-Wu PANG Qian XU Lu-Lai XU Yue-Song XU Peng-Wei and YANG Xin |
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| Institution: | Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University,Sichuan Academy of Grassland Science,Sichuan Academy of Grassland Science,Sichuan Academy of Grassland Science,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University and Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University |
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| Abstract: | The samples suspected infection of FMDV were identified by RT PCR method based on VP1 gene. One of the positive samples was sequenced and applied to homologous analysis with other reference sequences, to construct phylogenetic tree of VP1 gene. The results showed that the isolated VP1 belonged to serotype A, Asia topological type, and Sea 97 lineage. It has a high sequence similarity (99.1%) to A/GDMM/CHA/2013, a Chinese A type FMDV isolated in 2013, whereas has a low sequence similarity to the vaccine strain AF/72 (79.0%). The low sequence similarity between vaccine strain AF/72 and the A type FMDV viruses reported recently indicated that AF/72 vaccine strain might only provide limited protection and the vaccine strain should be made up to date as soon as possible. |
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| Keywords: | Foot and mouth disease virus VP1 Serotype A Phylogenetic analysis |
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