转EPSPS基因抗草苷膦油菜基因漂移的初步研究

草苷膦是一种广谱内吸传导型除草剂1].草苷膦通过竞争性抑制植物莽草酸代谢过程中磷酸烯醇式丙酮酸莽草酸-3-磷酸合酶(简称为EPSPS)活性,阻断芳香族氨基酸的生物合成导致植物死亡2].因此,转EPSPS基因(来自微生物的基因)作物对草苷膦类型的除草剂具有抗性.在1996~2004年的9年间,抗除草剂生物技术产品一直占市场的主导地位.2004年,全球种植的抗除草剂的转基因作物有油菜、大豆、玉米及棉花等,种植面积共计有5,860万公顷,占全球转基因作物总种植面积(8,100万公顷)的72%.抗除草剂油菜品种在国外已广泛种植,据国际农业生物技术应用服务组…

Preliminary Study on the EPSPS Gene from Glyphosate Resistant Brassica napus Move to Wild Brassica napus and Orychophragmus violaceus

The study used glyphosate resistant Brassica napus,planted in the field of Mianyang Normal College.The glyphosate resistant Brassica napus seeds wereplanted in the middle field,and the wild Brassica napus and Orychophragmus Violaceus were planted around the glyphosate resistant Brassica napus,in different directions in nature environment in 25 meters.The second year the wild Brassica napus and Orychophragmus Violaceus seeds were plantde in the field,and sprayed more glyphosate on the plants,and selected the plants which were not died.The leaves of not died wild Brassica napus were checked in the lab by PCR and Southern blot.The result indicated that four wild Brassica napus plant got the EPSPS gene from the glyphosate resistant Brassica napus,there are no gene flow between Orychophragmus Violaceus and glyphosate resistant Brassica napus.