| 药材甲热激蛋白基因SpHsp60的克隆及温度胁迫下的表达 |
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| 引用本文： | 朱晓晔,丁天波,许抗抗,严毅,李灿,杨文佳.药材甲热激蛋白基因SpHsp60的克隆及温度胁迫下的表达[J].四川大学学报(自然科学版),2017,54(6):1311-1316. |
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| 作者姓名： | 朱晓晔 丁天波 许抗抗 严毅 李灿 杨文佳 |
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| 作者单位： | 贵州师范大学 贵阳学院,青岛农业大学,贵阳学院,贵阳学院,贵阳学院,贵阳学院 |
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| 基金项目： | 国家自然科学基金青年基金项目（31501649），贵州省高层次创新型人才培养（黔科合人才[2016]4020）；贵州省科学技术联合基金项目（黔科合LH字[2014]7167）；贵阳学院高层次人才科研启动费（校人才2014003） |
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| 摘 要： | 本研究采用RT-PCR技术克隆了药材甲热激蛋白60(Heat shock protein 60,Hsp60)基因的cDNA全长序列,命名为SpHsp60(GenBank登录号:KX778623).氨基酸序列分析表明SpHsp60具有Hsp60基因家族高度保守的基序,实时定量PCR技术检测了不同温度胁迫后该基因的表达量,结果表明:-5℃和0℃低温处理2h,药材甲成虫体内的SpHsp60的表达量均显著高于对照组,且42℃高温处理2h后的表达量也显著高于对照组.说明SpHSP60与药材甲应对极端温度胁迫相关.
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| 关 键 词： | 药材甲 热激蛋白60 基因克隆 温度胁迫 表达模式 |
| 收稿时间： | 2016/11/21 0:00:00 |
| 修稿时间： | 2016/12/23 0:00:00 |
cDNA cloning of heat shock protein 60 (SpHsp60) in Stegobium paniceum and its expression under temperature stress |
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| ZHU Xiao-Ye,DING Tian-Bo,XU Kang-Kang,YAN Yi,LI Can and YANG Wen-Jia.cDNA cloning of heat shock protein 60 (SpHsp60) in Stegobium paniceum and its expression under temperature stress[J].Journal of Sichuan University (Natural Science Edition),2017,54(6):1311-1316. |
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| Authors: | ZHU Xiao-Ye DING Tian-Bo XU Kang-Kang YAN Yi LI Can and YANG Wen-Jia |
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| Institution: | College of Life Science, Guizhou Normal University; Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University,College of Plant Health and Medicine, Qingdao Agricultural University,Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University,Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University,Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University and Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University |
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| Abstract: | The full-length cDNA sequence of a heat shock protein 60 (Hsp60) gene from Stegobium paniceum (a major insect pest in stored Chinese medicine material) was cloned by using RT-PCR technique, and termed as SpHsp60 (GenBank accession no. KX778623). Sequence analysis showed that SpHsp60 contained an open reading frame of 1719 bp encoding 572 amino acids, with a predicated molecular weight of 60.9 kDa and isoelectric point of 5.32. SpHsp60 had the highly conserved functional motifs of the Hsp60 gene family, and SpHsp60 shares 88% amino acid sequence identities with the Hsp60 proteins from Tribolium castaneum and Leptinotarsa decemlineata. The quantitative real-time PCR results indicated that the expression of SpHsp60 was significantly increased 2 hours after exposed to -5°C and 0°C, compared to the control, and this gene was also induced after exposed to 42°C. The results suggest that the SpHsp60 gene may correlate with extreme temperature-stress tolerance in S. paniceum. |
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| Keywords: | Stegobium paniceum Heat shock protein 60 Gene cloning Temperature stress Expression pattern |
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