| 苏云金芽孢杆菌猝倒亚种Cry1Aa13基因的克隆及序列分析 |
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| 引用本文： | 钟万芳,吴洁,蔡平钟,阎文昭.苏云金芽孢杆菌猝倒亚种Cry1Aa13基因的克隆及序列分析[J].四川大学学报(自然科学版),2004,41(5):1050-1053. |
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| 作者姓名： | 钟万芳 吴洁 蔡平钟 阎文昭 |
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| 作者单位： | 四川省农业科学院生物技术育种中心,成都,610066;四川省农业科学院生物技术核技术研究所,成都,610066 |
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| 基金项目： | 973项目子课题(绿色农药的抗性变构靶标及活性测定新方法);四川省生物技术项目 |
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| 摘 要： | 根据Gen Bank中Cry1A类基因序列设计一对特异性引物，以苏云金芽孢杆茵猝倒亚种质粒DNA为模板，应用PCR扩增技术得到一大小约为3．6kb的DNA片段(Cry1Aa13)．通过引物步行法测定该片段长3598bp，其中开放阅读框(ORF)长3540bp，编码1180个氨基酸，分子量为133．49kD，等电点pI=5．0．序列比较表明该基因与Cry1Aa类基因高度同源(达95％以上)，该基因已在GenBank中登录，登录号为AF510713，并被Btδ-内毒素基因国际命名委员会正式命名为Cry1Aa13。
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| 关 键 词： | 苏云金芽孢杆菌猝倒亚种 Cry1Aa13 PCR 克隆 序列分析 |
| 文章编号： | 0490-0756(2004)05-1050-04 |
Cloning and Sequencing of Cry1Aa13 Gene from Bacillus thuringiensis subsp sotto |
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| ZHONG Wan-fang.Cloning and Sequencing of Cry1Aa13 Gene from Bacillus thuringiensis subsp sotto[J].Journal of Sichuan University (Natural Science Edition),2004,41(5):1050-1053. |
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| Authors: | ZHONG Wan-fang |
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| Institution: | ZHONG Wan-fang~ |
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| Abstract: | Cry1Aa13 gene had been cloned by PCR procedure from Bacillus thuringiensis subsp sotto plasmids with the primers designed according to the Cry1A sequence in the GenBank. Sequencing was done to the fragments using primer walking method and its length is 3598bp. Cry1Aa13 contained an 3540bp ORF that predicted a protein of 1180 amino acid with Mr=133.49kD and pI=5.0. The amino acid sequence of Cry1Aa13 gene shows more than 95% identity to other Cry1Aa, and the sequence was registered in GenBank(Accession Number is AF510713) which was designated Cry1Aa13 gene as a novel gene by Bt delta-endotoxin nomenclature committee. |
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| Keywords: | Bacillus thuringiensis subsp sotto Cry1Aa13 PCR cloning sequence analysis |
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