| 鸡白痢沙门氏菌直接-多重PCR检测体系的建立 |
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| 引用本文： | 程玲玲,严伟,侯若彤,帅培强,张彪,白林含.鸡白痢沙门氏菌直接-多重PCR检测体系的建立[J].四川大学学报(自然科学版),2019,56(1):149-154. |
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| 作者姓名： | 程玲玲 严伟 侯若彤 帅培强 张彪 白林含 |
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| 作者单位： | 四川出入境检验检疫局检验检疫技术中心,四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室,四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室,四川出入境检验检疫局检验检疫技术中心,成都市食品药品检验研究院,四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室 |
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| 基金项目： | 国家蛋鸡产业技术体系岗位科学家项目（CARS-41-K09） |
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| 摘 要： | 通过对鸡白痢沙门氏菌毒力质粒pSPUV上入侵质粒抗原J蛋白和质粒共转移调控因子的编码基因ipaJ和traJ的序列分析,在特异区域设计PCR引物,经沙门氏菌属不同种及常见肠道致病菌的交叉验证,筛选出三对鸡白痢沙门氏菌特异检测引物ipaJ-417、traJ-387、traJ-476.并且调试出一种利用两种DNA聚合酶的直接三重PCR检测体系:invA-211/traJ-387/16S-495,可以对鸡白痢沙门氏菌进行快速准确的鉴定.
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| 关 键 词： | 鸡白痢沙门氏菌 毒力质粒 pSPUV 特异检测引物 DNA聚合酶共用反应缓冲液 直接三重PCR检测体系 |
| 收稿时间： | 2017/9/29 0:00:00 |
| 修稿时间： | 2018/8/2 0:00:00 |
Establishment of a direct multiplex PCR detection system of Salmonella pullorum |
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| CHEN Ling-Ling,YAN Wei,HOU Ruo-Tong,SHUAI Pei-Qiang,ZHANG Biao and BAI Lin-Han.Establishment of a direct multiplex PCR detection system of Salmonella pullorum[J].Journal of Sichuan University (Natural Science Edition),2019,56(1):149-154. |
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| Authors: | CHEN Ling-Ling YAN Wei HOU Ruo-Tong SHUAI Pei-Qiang ZHANG Biao and BAI Lin-Han |
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| Institution: | lnspection and Quarantine Technical Center of Sichuan Entry-exit lnspection and Quarantine Bureau,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University,lnspection and Quarantine Technical Center of Sichuan Entry-exit lnspection and Quarantine Bureau,Chengdu Institutes for Food and Drug Control,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University |
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| Abstract: | In this paper, we screened out three pairs of specific detection primers of Salmonella pullorum, ipaJ 417, traJ-387, and traJ-476, by the blast analysis of the coding genes ipaJ and traJ which coded the invasion plasmid antigen protein J and plasmid co-transfer regulatory factor in Salmonella pullorum virulence plasmid Pspuv. And these PCR primers were designed in special regions and identificated in the different species of Salmonella and common intestinal pathogens. And a common reaction buffer of two kinds of DNA polymerase for the direct triple PCR system was also established (invA-211/traJ-387/16S-495). This direct triple PCR system can be used for fast and accurate identification of Salmonella pullorum. |
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| Keywords: | Salmonella pullorum Virulence plasmid pSPUV Specific detection primers Common reaction buffer of DNA polymerase Direct triple PCR system |
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