水葫芦化感物质N-苯基-2-萘胺对铜绿微囊藻生长的影响

摘 要：以不同浓度的N-苯基-2-萘胺对铜绿微囊藻进行处理，研究铜绿微囊藻的生长曲线、叶绿素a的含量和抗氧化酶的活性.结果表明，N-苯基-2-萘胺对铜绿微囊藻的抑制效果比较明显，EC50,7d=5 mg/L.7 mg/L和10 mg/L的N-苯基-2-萘胺培养液在第10天抑制效果分别达到92.7%和96.8%.叶绿素a的含量随着培养液中N-苯基-2-萘胺浓度的升高而降低，在10 mg/L的培养液中，培养时间8 h和24 h的藻叶绿素a的含量与对照相比分别降低了67.4%和75.9% .超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性都随着N-苯基-2-萘胺浓度的升高呈现先上升后下降的趋势，低浓度表现出促进作用，高浓度则表现出抑制作用.

Effects of allelochemical N-phenyl-2-naphthylamine from Water hyacinth on the growth of Microcystis aeruginosa

Abstract: The growth inhibition of allelochemical N-phenyl-2-naphthylamine isolated from roots of water hyacinth on the growth of Microcystis aeruginosa was investigated by determining chlorophyll a and antioxidant enzyme activity to understand the inhibition mechanism of N-phenyl -2-naphthylamine on M.aeruginosa .The result indicated that N-phenyl-2-naphthylamine had significant inhibitory effect on the growth of M .aerugim and the value of EC50,7d was 5 mg/L. the inhibition effects of medium supplied with 7 mg/L and 10 mg/L N-phenyl-2-naphthylamine nutritive medium were 92.7 % and 96.8 % respectively compared with the control. The content of chlorophyll a declined with the rise of N- phenyl-2-naphthylamine concentration. When the concentration of N-phenyl-2-naphthylamine is 10mg/L ,the content of chlorophyll a declined to 32.6% in 8h, and 24.1% in 24h. Activities of superoxide dismutase , peroxide and catalase were promoted by lower concentration of N-phenyl-2-naphthylamine, and lowered by higher concentration.