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| 仙客来(Cyclamen persicum Mill)体细胞胚的发生及组织细胞学的研究 | |
| 引用本文： | 由翠荣,樊廷俊,曲复宁,卞福花,梁丽琨,龚雪芹.仙客来(Cyclamen persicum Mill)体细胞胚的发生及组织细胞学的研究[J].四川大学学报(自然科学版),2008,45(6). |
| 作者姓名： | 由翠荣 樊廷俊 曲复宁 卞福花 梁丽琨 龚雪芹 |
| 作者单位： | 1. 中国海洋大学生命科学与技术学部海洋生命学院,青岛,266003;烟台大学化学生物理工学院,烟台,264005 2. 中国海洋大学生命科学与技术学部海洋生命学院,青岛,266003 3. 烟台大学化学生物理工学院,烟台,264005 |
| 基金项目： | 山东省科技厅科技公关项目 |
| 摘 要： | 以仙客来开花植株新生叶片为外植体,成功诱导、筛选出体细胞胚发生的胚性愈伤组织,胚性愈伤组织的形态有两种:微棕红色松软愈伤和松脆愈伤.显微镜下观察松软胚性愈伤多为含有数十个胚性细胞的胚性细胞团,而松脆胚性愈伤的胚性细胞团的胚性细胞数目只有几个或十几个.细胞学观察二者均包含前胚性细胞团、二细胞、四细胞、八细胞,进一步继代培养2周可观察到多细胞原胚、球形胚、梨形胚、心形胚、鱼雷胚的出现,大多数体胚60～90 d可发育成完整的小植株. |
| 关 键 词： | 仙客来 胚性愈伤组织 体细胞胚 组织细胞学 |
Somatic embryogenesis and study on histology and cytology in Cyclamen persicum Mill |
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| YOU Cui-Rong,FAN Ting-Jun,QU Fu-Ning,BIAN Fu-Hua,LIANG Li-Kun,GONG Xue-Qin.Somatic embryogenesis and study on histology and cytology in Cyclamen persicum Mill[J].Journal of Sichuan University (Natural Science Edition),2008,45(6). | |
| Authors: | YOU Cui-Rong FAN Ting-Jun QU Fu-Ning BIAN Fu-Hua LIANG Li-Kun GONG Xue-Qin |
| Institution: | YOU Cui-Rong1,2,FAN Ting-Jun1,QU Fu-Ning2,BIAN Fu-Hua2,LIANG Li-Kun2,GONG Xue-Qin2(1.College of Marine Life Science,Division of Life Science , Technology,Ocean University of China,Qingdao 266003,China,2.Scientific , Technological College of Chemistry , Biology,Yantai 264005,China) |
| Abstract: | Embryogenic calluses were successfully induced and filtrated from young leaf and petiole explants from flowering cyclamen plant,and two different embryogenic calluses were observed: one was slightly brown red and soft callus and the other was slightly brown red,soft and friable callus.The former mostly comprised of embryogenic clusters with several scores or more than one hundred embryogenic cells,while the latter only included in several or more than ten embryogenic cells.The two embryogenic calluses were ... |
| Keywords: | Cyclamen persicum Mill embryogenic callus somatic embryogenesis histology and cytology |
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