甘蓝型油菜角果和种子发育相关的Bna-novel-miR432功能分析

为了探究miRNA在油菜花器官发育、角果和种子发育中的功能，本研究在甘蓝型油菜不同发育阶段角果和种子小RNA测序数据分析中，筛选到一个新的miRNA，命名为Bna-novel-miR432，并将其转化至拟南芥中进行功能分析.研究发现过表达Bna-novel-miR432拟南芥开花时间显著提前，并且发现其雄蕊发育存在缺陷，导致雌蕊授粉不完全. 在对其花粉育性进行检测时发现过表达材料花粉育性显著降低. 对角果发育统计发现，过表达拟南芥株系的角果变短，角果中种子数量显著减少，且部分角果存在败育的现象. 此外，在种子发育方面，过表达材料种子中胚的发育也出现滞后的现象. 以上结果表明，Bna-novel-miR432参与调控植物开花时间、雄蕊和花粉的发育以及果实的形成等生物学过程中发挥一定的调控功能.

Functional analysis of Bna-novel-miR432 related to silique and seed development in Brassica napus

To explore the functions of miRNA in flower organ development, silique and seed development in Brassica napus, a novel miRNA named Bna-novel-miR432 was screened by analyzing the small RNA sequencing data of Brassica napus silique and seed at a different developmental stage. Then the miRNA was overexpressed in the Arabidopsis thaliana for functional analysis. The results showed that the flowering time of the stably transformed plants overexpressing Bna-novel-miR432 was significantly advanced, and the observation of flower development showed that there were defects in stamen development, resulting in incomplete pollination of the pistils. we further tested pollen fertility and found that the pollen fertility decreased significantly. The statistics of silique development showed that the length of silique in the overexpressed Arabidopsis lines was shorter, the number of seeds was significantly reduced and some of them were aborted. And the statistics on seeds development also showed that the development of the embryo was delayed. Therefore, Bna-novel-miR432 is involved in the regulation of flowering time, the development of stamens and pollen, and the development of fruit.