| 玉竹(Polygonatum odoratum(Mill.) Druce)凝集素的荧光淬灭研究 |
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| 引用本文： | 吕鸿周,刘超,鲍锦库.玉竹(Polygonatum odoratum(Mill.) Druce)凝集素的荧光淬灭研究[J].四川大学学报(自然科学版),2006,43(6):1389-1393. |
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| 作者姓名： | 吕鸿周 刘超 鲍锦库 |
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| 作者单位： | 四川大学生命科学学院,成都,610064 |
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| 基金项目： | 国家自然科学基金(30270331) |
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| 摘 要： | 百合科植物玉竹(Polygonatum odoratum(Mill.)Druce)的根状茎经生理盐水浸取、硫酸铵分级沉淀、CM-Sepharose柱离子交换层析和Sephacryl S-200凝胶过滤,得到经SDS-PAGE检测为14.2kD的单一蛋白条带的玉竹凝集素(Polygonatum odoratumlectin,简称POL).荧光淬灭剂丙烯酰胺、KI和CsCl对POL的内源荧光淬灭研究表明它们对凝集素分子荧光的淬灭过程属动态淬灭机制.其中玉竹凝集素中Trp残基对中性淬灭剂丙烯酰胺的可接近程度达到
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| 关 键 词： | 玉竹凝集素 荧光淬灭剂 荧光淬灭 |
| 文章编号： | 0490-6756（2006）06-1389-05 |
| 收稿时间： | 2006-03-27 |
| 修稿时间： | 2006-03-27 |
The Fluorescence Quenching of Polygonatum odoratum (Mill.)Druce Lectin |
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| LV Hong-Zhou,LIU Chao,BAO Jin-Ku.The Fluorescence Quenching of Polygonatum odoratum (Mill.)Druce Lectin[J].Journal of Sichuan University (Natural Science Edition),2006,43(6):1389-1393. |
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| Authors: | LV Hong-Zhou LIU Chao BAO Jin-Ku |
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| Institution: | College of Life Science; Sichuan University,College of Life Science; Sichuan University,College of Life Science; Sichuan University |
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| Abstract: | Polygonatum odoratum(Mill.) Druce lectin(POL) was isolated from Polygonatum odoratum((Mill.))Druce by extraction,fraction with(NH_(4))_(2)SO_(4,)ion exchange chromatography on CM-Sepharose column and followed by gel filtration on Sephacryl S-200.The purified POL shows a single band on SDS-PAGE of(14.2 kDa.)Fluorescence quenching study of POL by acrylamide, KI and CsCl suggested that they canquench 81%,50% and 45% of the intrinsic fluorescence of tryptophan in POL respectively,which indicated thatacrylamide |
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| Keywords: | Polygonatum odoratum(Mill ) Druce lectin fluorescence quenching quencher |
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