云芝多糖影响人宫颈癌HeLa细胞的增殖和凋亡的研究

研究云芝多糖(CVP)对宫颈癌HeLa细胞系的增殖、凋亡及产生途径。采用MTT法测定了CVP对HeLa细胞增殖的体外抑制作用，流式细胞仪检测凋亡细胞的比例，qRT-PCR检测P53、Bcl-2和Fas基因在细胞处理前后表达量的变化。结果显示：CVP以时间和剂量依赖的方式抑制HeLa细胞的生长。作用时间在24 h，48 h和72 h时对细胞抑制的抑制率分别为92%、95%和98%；当CVP浓度达到1.25 mg/L时，作用24 h、48 h和72 h时的IC50值分别为0.2507±0.01 mg/L、0.2720±0.04 mg/L和0.2736±0.03 mg/L；当CVP浓度为0.5 mg/L时，作用24 h和48 h后细胞凋亡率分别为26.36% 和63.81%；CVP处理HeLa细胞24 h后，Bcl-2基因的表达被显著下调。研究表明：CVP促进HeLa细胞凋亡，其作用机制与Bcl-2基因的表达下调有关，推测是通过Bcl-2介导的途径促进细胞凋亡。图4表1参30

Effects of Coriolus versicolor polysaccharides on proliferation and apoptosis of human cervical cancer HeLa cells in vitro

The objective of the current study was to explore the effect of Coriolus versicolor polysaccharides (CVP) on proliferation, apoptosis and its apoptosis pathway in cervical cancer HeLa cells. After HeLa cells was cultured. Cell viabilities were determined by the MTT method; the cell apoptosis rate was counted by by flowcytometer; The changes in expression level of P53, Bcl-2 and Fas gene before/after the cell treatment were detected by qRT-PCR. The proliferation of Hela cells was obviously inhibited by CVP in a does/time-dependent manner. When CVP treated cervical cancer HeLa cells for 24 h, 48 h and 72 h, the rates of CVP inhibition of cell growth was 92%, 95% and 98%, respectively. When CVP concentration reached to 1.25 mg/L, the effects on inhibition of HeLa cell growth were not significant difference with the increasing CVP concentration. While IC50 value of HeLa cell treated with CVP for 24, 48 and 72 h was 0.2507±0.01 mg/L, 0.2720±0.04 mg/L and 0.2736±0.03 mg/L, respectively. HeLa cells that were treated with CVP in 0.5 mg/L for 24 h, 26.36% of the cells had apoptosis, while for 48 h, 63.81% of the cells had apoptosis. After HeLa cells were treated with CVP for 24 h, the results of qRT-PCR analyses showed that the expression of Bcl-2 gene in the treated HeLa cells was down-regulated significantly. CVP could promote apoptosis of HeLa cells, and the mechanism may be associated with the down-regulation of Bcl-2 expression. HeLa cell apoptosis is supposed via Bcl-2 -mediated apoptotic pathway.