| 产胶原酶地衣芽孢杆菌菌种的分离、筛选及发酵条件研究 |
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| 引用本文： | 金敏,王忠彦,胡承,袁铸,胡永松.产胶原酶地衣芽孢杆菌菌种的分离、筛选及发酵条件研究[J].四川大学学报(自然科学版),2000,37(5):764-767. |
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| 作者姓名： | 金敏 王忠彦 胡承 袁铸 胡永松 |
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| 作者单位： | 四川大学生命科学学院,成都,610064 |
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| 摘 要： | 从成都皮革厂等处采集的样品中，分离筛选到一株胶原酶的产生菌Ｊ－４－８，菌种鉴定为地芽孢杆菌（Ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）。该菌产酶的最适碳源为蔗糖，氮源为明胶，超始ｐＨ为７．５，容瓶装量为１０ｍＬ，种龄是２４ｈ，接种量为６％，在此条件下，摇瓶振荡发酵后，酶活达２５Ｕ／ｍＬ发酵液，较原发酵培养基发酵后的酶活提高３５％。
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| 关 键 词： | 胶原酶 地衣芽孢杆菌 菌种 分离 筛选 发酵 药物 |
| 文章编号： | 0490-6756(2000)05-0764-04 |
STUDES ON THE BACILLUS LICHENIFORMIS SCREENING OF COLLAGENASE STRAIN AND THE CONDmONS FOR ENZYME PRODUCTION |
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| JIN Min,WANG Zong-Yan,HU Cheng,YUAN Zhu,HU Ying-song.STUDES ON THE BACILLUS LICHENIFORMIS SCREENING OF COLLAGENASE STRAIN AND THE CONDmONS FOR ENZYME PRODUCTION[J].Journal of Sichuan University (Natural Science Edition),2000,37(5):764-767. |
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| Authors: | JIN Min WANG Zong-Yan HU Cheng YUAN Zhu HU Ying-song |
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| Abstract: | A strain J 4 8 with yield of collagerase was obtained from 70 strains from soil samples.The optimou conditions for the enzyine production as follows: carbonic resource, sucrose; nitric resource, gelatin; initial pH 7.5; medium volumes 10ml; inoculum 6%;seeds age 24h. Cultivating 48h under these conditions, enzyme activity reaehed was 25U/mL of fermentation liquor,improved 35% compared with initial fermentation culture medium. |
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| Keywords: | Bacillus licheniformis collagenase enzyme activity |
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