| 一种适用于盐生植物的RNA提取方法 |
| |
| 引用本文： | 方孝东,吴多桂.一种适用于盐生植物的RNA提取方法[J].海南大学学报(自然科学版),1998,16(4):311-313. |
| |
| 作者姓名： | 方孝东 吴多桂 |
| |
| 作者单位： | 海南大学生物科学技术研究所 |
| |
| 基金项目： | 国家计委“九五”重点科技攻关项目 |
| |
| 摘 要： | 介绍了一种红树ＲＮＡ提取方法，此法无需特殊试剂和贵重仪器设备，可有效地排除红树细胞中大量存在的胶质和多糖类物质的干扰，所得ＲＮＡ样品经紫外分光光度计检测和１％琼脂糖凝胶电泳分析证明具有较高的纯度和完整性，可满足多数分子生物学实验的需要，尤其适用于提取多糖含量较高的植物细胞的ＲＮＡ．
|
| 关 键 词： | 核糖核酸 提取 红树 |
A Suitable Method for the RNA Extraction of Halophyte |
| |
| Fang Xiaodong,Wu Duogui,Lin Qifeng,Li Guanyi.A Suitable Method for the RNA Extraction of Halophyte[J].Natural Science Journal of Hainan University,1998,16(4):311-313. |
| |
| Authors: | Fang Xiaodong Wu Duogui Lin Qifeng Li Guanyi |
| |
| Abstract: | A simple extraction method for the total RNA of Rhizophora apiculata is introduced here.This method requires no special reagent and valuable instrument.It can efficiently eliminate the interferene of the polysaccharide which is very rich in Rhizophora apiculata. The results of 1% agarose gel electrophoresis and ultraviolet spectrophotometer analysis show that the total RNA of Rhizophora apiculata extracted through this method has no obvious degradation and has a good purity and can meet the needs of most molecular biological experiments.It is especially useful for the RNA extraction of plant cell which usually has plenty of polysaccharide. |
| |
| Keywords: | RNA extraction Rhizophora apiculata |
| 本文献已被 CNKI 维普 等数据库收录！ |
