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Elf-1基因在急性髓细胞性白血病中的表达情况
引用本文:沈琦,郑海涛,陈少华,杨力建,李扬秋.Elf-1基因在急性髓细胞性白血病中的表达情况[J].暨南大学学报,2009,30(6).
作者姓名:沈琦  郑海涛  陈少华  杨力建  李扬秋
作者单位:1. 暨南大学医学院血液病研究所,广东,广州,510632
2. 暨南大学医学院血液病研究所,广东,广州,510632;暨南大学再生医学教育部重点实验室,广东,广州,510632
基金项目:国家自然科学基金项目 
摘    要:目的:建立实时定量PCR检测Elf-1基因表达水平的方法,了解急性髓细胞性白血病(AML)患者外周血Elf-1基因表达水平.方法:采用SYBR Green Ⅰ荧光定量PCR和相对定量分析法检测33例AML患者:M2型17例、M3型6例、M5型10例和20例健康人外周血的单核细胞的Elf-1表达情况,以β2微球蛋白基因(β2M)作为内参,采用公式2~(-△Ct)×100%计算Elf-1基因表达水平.结果:AML组Elf-1表达水平(13.518±19.197)%明显高于健康对照组(2.044±1.321)%(P<0.01),不同AML亚型之间Elf-1的表达水平没有显著性差异(P=0.52),但各亚型AML的Elf-1的表达水平均与健康对照组比较均有显著性差异(P<0.01).结论:建立SYBR Green Ⅰ荧光实时定量PCR分析外周血Elf-1表达水平的方法,检测急性髓细胞性白血病Elf-1基因高表达情况.

关 键 词:Elf-1基因  实时定量PCR  急性髓细胞性白血病(AML)  T细胞免疫缺陷  TCRζ链  TCR  ζ  chain

The feature of Elf-1 gene expression in acute myeloid leukemia
SHEN Qi,ZHENG Hai-tao,CHEN Shao-hua,YANG Li-jian,LI Yang-qiu.The feature of Elf-1 gene expression in acute myeloid leukemia[J].Journal of Jinan University(Natural Science & Medicine Edition),2009,30(6).
Authors:SHEN Qi  ZHENG Hai-tao  CHEN Shao-hua  YANG Li-jian  LI Yang-qiu
Abstract:Aim:To establish a real-time PCR technique for detection and quantify of Elf-1 gene ex-pression and to investigate the expression level of Elf-1 gene in patients with acute myeloid leukemia (AML). Methods: Real-time PCR with SYBR Green Ⅰ technique was used for detecting Elf-1 expres-sion level in peripheral blood mononuclear cells of 33 patients with AML (including 17 cases with M2, 6 cases with M3, 10 cases with M5 and 20 healthy individuals). β2-microglobulin gene (β2M) was used as an endogenous reference. Relative changes in Elf-1 expression level were used by the 2~(-△Ct)×100% method. Results: The expression level of Elf-1 in 33 cases with AML(13.518±19.197%) was signifi-cant higher than those in the healthy control (2.044±1.321% ) (P<0.01). The expression level of Elf-1 had not significant difference among the three subtypes (P=0.52). However, by comparing with the healthy control, the expression level of Elf-1 had significant difference in all of three subtypes (P< 0.01 ). Conclusion:The SYBR Green Ⅰ real-time technique for quantitative detection of Elf-1 expression levels was established. And the overexpression of Elf-1 gene in AML was detected.
Keywords:Elf-1 gene  real-time PCR  acute myeloid leukemia(AML)  T-cells immunodefi-ciency
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