牛乳清蛋白BALB/c小鼠过敏模型的建立及其优化

目的:建立牛乳清蛋白的BALB/c小鼠过敏模型,并探讨不同佐剂、免疫途径及饲料纯度等因素对建立牛乳清蛋白的BALB/c小鼠过敏模型的影响。方法:用不含牛乳及乳制品的特制饲料饲养BALB/c小鼠以乳清蛋白致敏,间接ELISA法测定特异性IgE,用荧光法检测体外激发小鼠致敏肥大细胞释放的组胺。结果:成功建立BALB/c小鼠乳清蛋白过敏模型,条件优化结果显示,氢氧化铝佐剂结合皮下注射最好地促进特异性IgE产生;用不含牛奶及其他食物过敏原的特制饲料饲养的小鼠特异性IgE的本底水平低,适于建立小鼠过敏模型,荧光法检测肥大细胞释放组胺的结果与特异性IgE检测结果一致。结论:用特制饲料饲养小鼠,氢氧化铝佐剂结合皮下注射乳清蛋白适于建立BALB/c小鼠过敏症模型,该模型可用于过敏症机理研究。

The experiment study of allergic models with BALB/c mice for whey protein

Aim: To establish allergic model with BALB/c mice for milk whey protein,this study explor the various conditions that influenced the establishment of the food allergic model,including different adjuvant,immunized approaches and feed,et al.Methods: The BALB/c mice bred for two generations on a milk-free diet,and then the allergic mice model was established with milk whey protein,the specific IgE against whey protein was detected by indirect ELISA and the releasing histamine of peritoneal mast cells was determined by fluorescence spectrophotomet.Results:Allergic BALB/c model of whey protein had been established remarkably by subcutaneous injected with the adjuvant of aluminum hydroxide.The base value of the BALB/c mice bred for at least two generations on a milk-free diet is low,which apply to establish allergic model.The results of releasing histamine ratio were well accordance with the results of the specific IgE.Conclusion: An allergic model of milk has been established by BALB/c subcutaneous injected whey protein with aluminum hydroxide as an adjuvant,which might become a new method to characterize and assess food allergens.