| 光密度法测定微藻生物量 |
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| 引用本文： | 沈萍萍,王朝晖,齐雨藻,谢隆处,王艳.光密度法测定微藻生物量[J].暨南大学学报,2001,22(3):115-119. |
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| 作者姓名： | 沈萍萍 王朝晖 齐雨藻 谢隆处 王艳 |
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| 作者单位： | 暨南大学水生生物研究所, |
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| 摘 要： | 目的：为准确而又快速的测量微藻生物量。方法：选用15种不同微藻，在实验室中分别测定其细胞密度及光密度并进行直线回归分析，同时采用吸光系数来估算浮游植物生物量。结果：得出了浮游植物光系数与细胞碳含量（即生物量）的回归方程：lg(m)=-1.0465ln(x) 4.2551.结论：这是一种利用光密度法来测量微藻生物量的简单有效的方法。
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| 关 键 词： | 微藻 吸光系数 生物量 碳含量 光密度法 细胞密度 含量测定 |
| 文章编号： | 1000-9965（2001）03-0115-05 |
| 修稿时间： | 2000年11月15 |
An optical density method for determination of microalgal biomass |
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| SHEN Ping-ping,\ WANG Zhao-hui,\ QI Yu-zao,\ XIE Long-chu,\ WANG Yan.An optical density method for determination of microalgal biomass[J].Journal of Jinan University(Natural Science & Medicine Edition),2001,22(3):115-119. |
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| Authors: | SHEN Ping-ping \ WANG Zhao-hui \ QI Yu-zao \ XIE Long-chu \ WANG Yan |
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| Abstract: | Aim: To investigate the relationship between optical density (OD) and the biomass of microalgae. Methods: 15 microalgal species were used under laboratory conditions to study the relationship between the cell density and its related OD. Meanwhile, the optical absorption coefficient, namely the slope of the linear graph which showed the relationship between cell density and OD, was utilized to estimate the phytoplankton biomass. Results: Regression analysis results showed the linear function: lg( m )=-0.451 1 ln( x )+4.242 2, in which c is the carbon content and x is the optical absorption coefficient of microalgae. Conclusion: This is a useful and rapid OD method to measure the biomass of microalgae . |
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| Keywords: | microalgae optical absorption coefficient biomass carbon content OD |
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