PCR技术检测金黄色葡萄球菌肠毒素D基因

根据金黄色葡萄球菌肠毒素Ｄ基因的序列，设计相应的引物，建立检测金黄色葡萄球菌肠毒素Ｄ基因的ＰＣＲ技术。利用该方法可从含有肠毒素Ｄ基因的金黄色葡萄球菌中扩增出ＰＣＲ产物。扩增产物具有特异性，长为３１６个碱基对。经限制性核酸内切酶ＨｈＩⅠ酶切证实扩增产物为ＳＥＤ基因片段。

Detection of the gene encoding Staphylococcal enterotoxin D by PCR

A PCR assay for detecting the gene encoding Staphylococcal enterotoxin D (SED) was developed . According to the sequences of the SED gene, primers were selected and were used to amplify SED gene. The length of amplicon was 316 bp. The amplified fragments were analyzed by digestion of restriction endonuclease HhI I. The results showed the PCR assay develpoed was a specific, sensitive, quick and efficient way of detecting SED gene. It provides a useful tool for the diagnosis of bacterial infection and the identification of microorganisms in food.