应用PCR技术检测结核菌的临床研究

应用聚合酶链反应（ＰＣＲ）技术对２４４例呼吸疾病患者临床标本进行结核菌ＤＮＡ检测，并同时与涂片抗酸染色结果进行比较。结果显示：１１２例结核标本涂片和ＰＣＲ检测阳性率分别为１６．１％和５２．７％，两者差别有显著性（Ｐ＜０．０１）。９４例涂阴结核标本ＰＣＲ阳性达４５．７％。结果表明应用ＰＣＲ技术检测结核杆菌ＤＮＡ具有快速敏感、特异性较高等优点，尤其对涂阴病人具有较大诊断价值

Application of Polymerase Chain Reaction to DetectMycobacterium Tuberculosis DNA

Polymerase chain reaction (PCR) was used to detect Mycobacterium tuberculosis DNA in examinations of 244 respiratory specimens. Results from PCR were compared with those of acid-fast stained smears or diagnosis. It was found that in 112 tuberculous speci-mens, the positive rates of acid-fast staining and PCR were 16.1% and 52.7% respectively. The sensitivity of PCR was much higher than that of acid-fast staining(P<0.01). It was still 45.7% that the positive rates of PCR in 94 negative acid-fast staining tuberculous samples. This data demonstrated that PCR method is useful for rapid and direct detection of M. tuberculosis, shows high sensitivity and specificity, and has a greater diagnositic value for the smear negative patients.