斑节对虾杆状病毒感染对脂肪代谢的影响

目的 :为了探究斑节对虾杆状病毒 (MBV)感染对脂肪代谢的影响 ,指导虾病的防治 .方法 :利用索氏 (Soxhlet)提取法对养殖健康斑节对虾和MBV感染斑节对虾的中肠腺脂肪含量进行了检测 .结果 :健康对虾中肠腺脂肪占干重平均 34 2 8% ,占湿重平均 9 60 % ,含水率平均 71 0 6% .MBV轻度感染的中肠腺脂肪占干重平均 31 52 % ,占湿重平均 9 58% ,含水率平均 69 31% ,三者与健康对虾比较均无显著性差异 (P >0 30、P >0 90和P >0 4 0 ) .MBV重度感染的中肠腺脂肪占干重平均 56 4 2 % ,占湿重平均 2 4 14% ,两者均明显高于健康虾和MBV轻度感染虾 (均为P <0 0 0 1) ;含水率平均 57 2 2 % ,明显低于健康虾和MBV轻度感染虾 (均为P <0 0 0 1) .结论 :严重感染MBV的斑节对虾可出现脂肪代谢紊乱 ,造成脂肪堆积 ,进而影响中肠腺的消化吸收功能

Influence on fat metabolism in mid-gut gland of healthy Penaeus monodon and that infected with Penaeus monodon baculovirus

Aim: To study the relationship between MBV infection and fatty metabolism. Method: The fat average levels of mid-gut gland in healthy Penaeus monodon and in P. monodon infected with Penaeus monodon baculovirus (MBV) were detected by Soxhlet method. Results: The fat average level was 34.28% of dry weight or 9.60% of wet weight and the water average content was 71.06% in healthy P. monodon . The fat average level was 31.52% of dry weight or 9.58% of wet weight and the water average content was 69.31% in P. monodon lightly infected with MBV. There was no distinct difference between healthy P. monodon and P. monodon lightly infected with MBV in fat level and water content ( P >0.30, P >0.90 and P >0.40). The fat average level was 56.42% of dry weight or 24.14% of wet weight and the water average content was 57.22% in P. monodon heavily infected with MBV. The fat level was distinctly higher ( P <0.001) and the water content was distinctly lower ( P <0.001) as compared with those in healthy P. monodon and that lightly infected with MBV. Conclusion: There was metabolic disorder in mid-gut gland heavily infected with MBV.