| HPLC法和FPIA法测定茶碱血清浓度的相关性 |
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| 引用本文： | 李俊杰,杨杲岚,陈琳.HPLC法和FPIA法测定茶碱血清浓度的相关性[J].暨南大学学报,2006,27(2):230-232. |
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| 作者姓名： | 李俊杰 杨杲岚 陈琳 |
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| 作者单位： | 暨南大学附属第一医院临床药理室,广东,广州,510630 |
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| 摘 要： | 目的:建立快速测定茶碱血清浓度的高效液相色谱(HPLC)法,研究应用此法和常规的荧光偏振免疫分析(FPIA)法测定结果的相关性。方法:分别用HPLC和FPIA法测定58份茶碱血清样品,比较两种方法的测定结果。结果:HPLC法(Y)和FPIA法(X)测定的茶碱血清浓度的回归方程为Y=0.282 5+1.064X(r=0.988 1)。r的t检验显示两种方法测定结果之间的直线相关关系有显著意义(P<0.001)。结论:用HPLC法和FPIA法测定的茶碱血清浓度具有良好的相关性;与FPIA法相比,该HPLC法不仅同样具有快速、简便和准确的特点,而且检测成本较低,也可作为监测茶碱血清浓度的常规方法。
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| 关 键 词： | 高效液相色谱 荧光偏振免疫分析 茶碱血清浓度 |
| 文章编号： | 1000-9965(2006)02-0230-03 |
| 修稿时间： | 2005年10月18 |
Research on relativity between serum theophylline concentrations determined by HPLC and by FPIA |
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| LI Jun-jie,YANG Gao-lan,CHEN Lin.Research on relativity between serum theophylline concentrations determined by HPLC and by FPIA[J].Journal of Jinan University(Natural Science & Medicine Edition),2006,27(2):230-232. |
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| Authors: | LI Jun-jie YANG Gao-lan CHEN Lin |
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| Abstract: | Aim: To establish a rapid HPLC method determining serum theophylline concentration and research the relativity of results determined by HPLC and by routine FPIA.Methods:Serum theophylline concentrations of 58 samples were determined by HPLC and by FPIA separately,and the results were compared.Results: The regression(equation) of serum theophylline concentrations determined by HPLC(Y) and by FPIA(X) was Y=0.282 5+1.064X(r=0.988 1).The t test of correlation coefficient showed significant linear correlation between the results of two methods(P<0.001).Conclusion: The relativity is good between the serum theophylline concentrations determined by HPLC and by FPIA.Compared with FPIA,the HPLC method is not only simple,rapid and accurate as well,but also cheap for the analysis cost.It can also be applied as the routine(method) to monitor serum theophylline concentration. |
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| Keywords: | HPLC FPIA serum theophylline concentration |
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