| 异亮氨酸对他克莫司发酵的影响 |
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| 引用本文： | 顾骏,叶蕊芳,赵晔惟,张燕,郑黎.异亮氨酸对他克莫司发酵的影响[J].华东理工大学学报(自然科学版),2007,33(3):323-326. |
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| 作者姓名： | 顾骏 叶蕊芳 赵晔惟 张燕 郑黎 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 摘 要： | 研究了前体氨基酸对发酵合成他克莫司的影响,结果显示:在发酵初期添加2.5 g/L的异亮氨酸(Ile),他克莫司产量提高约59%。有机酸分析和丙酰CoA合成酶与丙酰激酶活性测定的结果显示:异亮氨酸的加入显著增加了丙酸(Pro)的浓度,在产物合成期丙酰CoA合成酶的最大酶活提高到600 U/g。由此表明该前体氨基酸促进他克莫司发酵合成的原因可能是其促进了丙酰CoA等前体物质的合成,从而增进了内酯环的形成。
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| 关 键 词： | 异亮氨酸 他克莫司 有机酸 丙酰CoA合成酶 |
| 文章编号： | 1006-3080（2007）03-0323-04 |
| 修稿时间： | 2006-06-30 |
Effects of Isoleucine on Tacrolimus Biosynthesis |
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| GU Jun,YE Rui-fang,ZHAO Ye-wei,ZHANG Yan,ZHENG Li.Effects of Isoleucine on Tacrolimus Biosynthesis[J].Journal of East China University of Science and Technology,2007,33(3):323-326. |
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| Authors: | GU Jun YE Rui-fang ZHAO Ye-wei ZHANG Yan ZHENG Li |
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| Institution: | State Key Laboratory of Bioreactor Engineering;East China University of Science and Technology;Shanghai 200237;China |
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| Abstract: | Tacrolimus(FK506)is a novel immunosuppressant.The results of the influence of amino acid on tacrolimus production show that the yield of tacrolimus are increased by about 59% by adding 2.5 g/L(isoleucine(Ile)) on the initial stage of fermentation,while the concentration of the propionoic acid is greatly increased and the activity of propionyl-CoA synthetase is achieved to 600 U/g in the production formation phase.The reason for the increase of tacrolimus titer is that the synthesis of propionyl-CoA and othe... |
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| Keywords: | isoleucine tacrolimus organic acid propionyl-CoA synthetase |
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