| 以CER为判据从摇瓶到反应器的ATP放大生产 |
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| 引用本文： | 朱文洲,徐茜.以CER为判据从摇瓶到反应器的ATP放大生产[J].华东理工大学学报(自然科学版),1998,24(1):59-64. |
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| 作者姓名： | 朱文洲 徐茜 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室 |
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| 摘 要： | 在Ｙ－Ｚ型多功能摇瓶中由固定化啤酒酵母将腺苷转化成ＡＴＰ，得到一组过程参数曲线，以摇瓶转化过程中的ＣＯ２释放率（ＣＥＲ）－转化时间曲线为参照曲线，在液流循环式固定化啤酒哮母流化床反应器中进行放大试验。对比实验结果，表明以ＣＥＲ为判据进行固定化啤酒酵母生产ＡＴＰ从摇瓶到反应器的放大是成功的。
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| 关 键 词： | 摇瓶 放大判据 固定化酵母 ATP 酶 |
Study of ATP Production with Immobilized Saccharamyces Cerivisae Using Carbon Dioxide Evolution Rate as Scale up Criterion |
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| Zhu Wenzhou,Xu Qian,Qiu Weiran and Yu Juntang.Study of ATP Production with Immobilized Saccharamyces Cerivisae Using Carbon Dioxide Evolution Rate as Scale up Criterion[J].Journal of East China University of Science and Technology,1998,24(1):59-64. |
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| Authors: | Zhu Wenzhou Xu Qian Qiu Weiran and Yu Juntang |
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| Institution: | Zhu Wenzhou,Xu Qian,Qiu Weiran and Yu Juntang * |
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| Abstract: | The biotransformation of adenosine to ATP using immobilized saccharamyces cerivisae in multifunctional shake flask of Y Z type was studied and the process parameter curves were obtained. When the same process was scaled up into a liquid recycling fluidized bed reactor, it was found that of the time course profile of carbon dioxide evolution rates( CER ) was maintained identically with that obtained from sake flask test same conversion results could be obtained as that from shake flask test. So the CER can be considered as a sound scale up criterion of this biotransformation process. |
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| Keywords: | shake flask scale up criterion immobilized saccharamyces cerivisae ATP carbon dioxide evolution rate( CER ) |
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