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| 球等鞭金藻生长抑制物的分离提取与抑制活性 | |
| 引用本文： | 孙颖颖,王长海,陈静.球等鞭金藻生长抑制物的分离提取与抑制活性[J].华东理工大学学报(自然科学版),2007,33(3):340-344,368. |
| 作者姓名： | 孙颖颖 王长海 陈静 |
| 作者单位： | 1. 大连理工大学环境科学与生命学院,辽宁大连,116024 2. 大连理工大学环境科学与生命学院,辽宁大连,116024;烟台大学海洋学院,山东烟台,264005 3. 烟台大学海洋学院,山东烟台,264005 |
| 基金项目： | 国家“十五”科技攻关资助项目(2001BA707B03) |
| 摘 要： | 以含有生长抑制物的球等鞭金藻的微藻滤液为研究对象,考察pH、时间、料液体积比和溶剂等因素对生长抑制物提取效果的影响,确定了球等鞭金藻的微藻滤液中生长抑制物的适合提取工艺;通过比较两种型号的葡聚糖凝胶对生长抑制物粗提物的分离效果,确定了适合的凝胶类型;同时研究温度对生长抑制物抑制活性的影响以及生长抑制物对其他微藻生长的抑制作用。结果表明:生长抑制物的适宜提取工艺为pH 2~4、提取时间3 h、料液体积比10∶1、溶剂为乙酸乙酯;Sephadex G-15凝胶对生长抑制物的分离效果好于Sephadex G-25;生长抑制物的抑制活性具有很强的热稳定性,其对角毛藻和小球藻有明显的抑制作用。 |
| 关 键 词： | 球等鞭金藻 生长抑制物 提取 抑制活性 |
| 文章编号： | 1006-3080（2007）03-0340-05 |
| 修稿时间： | 2006-08-03 |
Extraction, Initial Purification and Inhibitory Activity of Growth-Inhibitor Formed by Isochrysis galbana |
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| SUN Ying-ying,WANG Chang-hai,CHEN Jing.Extraction, Initial Purification and Inhibitory Activity of Growth-Inhibitor Formed by Isochrysis galbana[J].Journal of East China University of Science and Technology,2007,33(3):340-344,368. | |
| Authors: | SUN Ying-ying WANG Chang-hai CHEN Jing |
| Institution: | 1;2;1.School of Bioscience and Biotechnology;Dalian University of Technology;Dalian 116024;Liaoning;China;2.School of Ocean;Yantai University;Yantai 264005;Shandong;China |
| Abstract: | The extraction process,initial purification and inhibitory activity of growthinhibitor that formed by Isochrysis galbana were investigated.And it was found that different factors impacting on(extraction) were as followed: pH,extraction time,the ratio of materials for extraction liquid and extraction solvent.Initial purification of growth-inhibitor was carried out by gel-filtered on Sephadex G column.The effect of temperature on the inhibitory activity of growth-inhibitor and the effect of growth-inhibitor o... |
| Keywords: | Isochrysis galbana growth-inhibitor extraction inhibitory activity |
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