| 猪血过氧化氢酶的纯化及其性质研究 |
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| 引用本文： | 袁勤生,洪麟,范玉兰.猪血过氧化氢酶的纯化及其性质研究[J].华东理工大学学报(自然科学版),1988(6). |
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| 作者姓名： | 袁勤生 洪麟 范玉兰 |
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| 作者单位： | 华东化工学院生化工程系
(袁勤生，洪麟)，华东化工学院生化工程系(范玉兰) |
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| 摘 要： | 采用乙醇-氯仿去血红蛋白、硫酸铵分级沉淀、CM-32 DEAE-Sephadex A-50柱层析和超滤等步骤,从猪血红细胞中获得了高纯度的超氧化物歧化酶(SOD)和过氧化氢酶(CTS),纯化后的CTS比活达7580u/mg,分子量为229 646。紫外检测CTS在280,405,505,535,583和620nm均有光吸收,经测定CTS有18种氨基酸组成,最适pH为7.0,K_m为22mmol,K_(cat)为0.18/min,金属离子对酶活性的影响与金属的浓度及所带电荷有关。
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| 关 键 词： | 生物制品 提纯 过氧化氢酶 牛肝 动物血 猪血红细胞 CTS的性质 |
Studies on purification and characteristics of hog erythrocyte catalase |
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| Yuan Qinsheng,Hong Lin Fan Yulan.Studies on purification and characteristics of hog erythrocyte catalase[J].Journal of East China University of Science and Technology,1988(6). |
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| Authors: | Yuan Qinsheng Hong Lin Fan Yulan |
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| Institution: | Department of Biochemical Engineering |
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| Abstract: | Purification of catalase from hog erythrocyte is described in this paper. SOD and CTS of high purity with specific activity 7580u/mg protein were obtained by removal of hemogiobin with chloroformethanol, chromatographic separation through CM-32 and DEAE-Sepha-dex A-50 column, ammonium sulfate precipitation and ultrafiltration. Molecular weight of approximate 229 646 was identified. The physicochemical characteristics of CTS such as absorption spectrum, amino acid composition, optimal pH, K_m, K_(cat), effect of metal ions, etc. were studies. |
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| Keywords: | biologicals purifications catalase bovine liver animalblood hog erythrocyte property of CTS |
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