| 酚类化合物酶处理反应机理初探 |
| |
| 引用本文： | 黄慧,赵庆祥.酚类化合物酶处理反应机理初探[J].华东理工大学学报(自然科学版),1998,24(5):563-568. |
| |
| 作者姓名： | 黄慧 赵庆祥 |
| |
| 作者单位： | 华东理工大学环境工程系 |
| |
| 摘 要： | 研究了辣根过氧化物酶处理酚和氯酚的反应机理和动力学特征。根据酶反应动力学特征判断辣根过氧化物酶催化酚和氯酚氧化耦合反应属于乒乓双双反应机制。阐明了Ｈ２Ｏ２抑制机理，测定了酶反应中Ｈ２Ｏ２抑制的动力学参数。通过测定不同底物酶反应的动力学参数，比较了取代基数目及取代位置对酶反应的影响。
|
| 关 键 词： | 酶反应机理 酚类化合物 造纸废水 废水处理 |
Study on the Mechanism of Phenols Removal from Pulp and Paper Wastewater Catalyzed by Horseradish Peroxidase |
| |
| Huang Hui ,Zhao Qingxiang,Zhang Tong and Li Qin.Study on the Mechanism of Phenols Removal from Pulp and Paper Wastewater Catalyzed by Horseradish Peroxidase[J].Journal of East China University of Science and Technology,1998,24(5):563-568. |
| |
| Authors: | Huang Hui Zhao Qingxiang Zhang Tong and Li Qin |
| |
| Institution: | Huang Hui *,Zhao Qingxiang,Zhang Tong and Li Qin |
| |
| Abstract: | The kinetics and mechanism of the enzymatic reaction of phenol and chlorophenol were studied in this paper. It was concluded that oxidative coupling reaction catalyzed by horseradish peroxidase had the characteristic of Ping Pang reaction. The effect of H 2O 2 concentration on the enzymatic reaction was discussed in detail. The results showed that H 2O 2 had an inhibition effect on phenols removal and the inhibition mechanism of H 2O 2 was elucidated. The removal reactions for several substrates were compared according to the kinetics parameters. It was shown that there was a definite relationship between the efficiency of enzymatic reaction and the amount and position of various substituted groups for substrates. |
| |
| Keywords: | horseradish peroxidase mechanism of enzymatic reaction phenol chlorophenol pulp and paper wastewater |
| 本文献已被 CNKI 维普 等数据库收录！ |
