连翘中具有药物活性成分内生菌的筛选及其拮抗特性的研究

采用组织分离法从连翘枝条中筛选出13株内生菌,其中有7株内生真菌,6株内生细菌。分别以金黄色葡萄球菌、大肠杆菌为指示菌株,采用平板对峙法对分离出的13株内生菌的抗菌活性进行筛选。结果表明：13株菌种中有10个菌株对1种供试菌具有抑菌活性。其中L-07-01、L-07-09、L-07-12、L-07-13的拮抗效果明显,L-07-09对金黄色葡萄球菌具有较强的抑菌效果,测量其抑菌圈直径为10 mm。L-07-12对大肠杆菌具有较强抑菌效果,测量其抑菌圈直径为10 mm。对拮抗效果较强的菌株进行液体发酵培养后采用双层打孔法,测定其抑菌效果后证明L-07-13对金黄色葡萄球菌有极强的抑制效果。对拮抗后的指示菌进一步镜检后,发现菌体有明显的溶菌、空洞、畸形等现象。连翘内生菌可以产生丰富的具有药物活性的次生代谢产物,可作为新型药物的筛选源。

Screening of Drug Active Products Endophytic from Fructus Forsythiae and Study on Their Antagonism

Thirteen strains of endophyte were isolated from Fructus forsythiae branches were screened by using organization method separation,including seven strains of endophytic fungi,six strains of endophytic bacteria. Respectively to Staphylococcus aureus,Escherichia coli as the indicator strain,on the isolated thirteen strains of endophytic bacteria antimicrobial activity was screened by plate confrontation method. The results showed that： thirteen strains in ten strains of one species of tested bacteria with antimicrobial activity. One obvious antagonistic effect of L- 07-01,L- 07- 09,L- 07- 12,L- 07- 13,L- 07- 09 has a strong inhibitory effect on Staphylococcus aureus,measuring the inhibition zone diameter was 10 mm. L- 07- 12 has a strong inhibitory effect on Escherichia coli,measuring the inhibition zone diameter was 10 mm. The antagonistic effect of strong strain liquid fermentation and culture after using double diffusion method,the determination of the antagonistic effect after demonstrated that L- 07- 13 has a strong inhibitory effect on Staphylococcus aureus. To further check of lens of indicator bacteria antagonistic after cells have obvious lysis,empty and abnormal. Fructus forsythiae endophyte can produce abundant secondary metabolites with activities,it can be used as a source of new drugs screening.