邻香草醛缩胱氨酸双Schiff碱的合成及其对粟酒裂殖酵母细胞生长代谢的热动力学研究

用摩尔比为2:1的邻香草醛(C_8H_8O_3)与L-胱氨酸(C_6H_(12)N_2O_4S_2)反应,合成了一种新的双Schiff碱化合物--双{2-(3-巯基丙酸钠)-2-亚胺基-甲基]-6-甲氧基-苯酚}(OVCS)。通过元素分析、红外光谱、核磁共振等手段对其组成和结构进行了表征,确定其化学式为Na_2(C_(22)H_(22)N_2O_8S_2),采用TAM air微量热仪测定了新合成的Schiff碱化合物(OVCS)在305.15 K时对粟酒裂殖酵母细胞作用的产热曲线;根据产热曲线计算了在OVCS作用下,粟酒裂殖酵母细胞生长代谢的最大发热功率p_(max)、速率常数k、传代时间tG、抑制率I和半抑制浓度C_(I,50)等热动力学参数。通过实验可以发现随着OVCS浓度的增加,粟酒裂殖酵母细胞的生长代谢速率常数k、生长代谢的总热效应Q_(total)、最大发热功率p_(max)均减小,抑制率I、达到生长代谢最大功率所需时间t_(max)、传代时间tG均增加等规律,半抑制浓度C_(I,50)为35.99 mg/L(或9.62×10~(-2)mol/L)。实验结果表明,OVCS对粟酒裂殖酵母细胞有抑制作用,且浓度越大,抑制作用越强。

SYNTHESIS AND THERMOKINETIC STUDY ON THE S.POMBE GROWTH METABOLISM OF A NOVEL BIS-SHCIFF BASE DERIVED FROM O-VANILLIN AND L-CYSTINE

A novel Schiff base named Bis{2-(3-mercapto propionic acid sodium-2-ylimino)-methyl]-6- methoxy-phenol}(abbreviated as OVCS) was synthesized using 2:1 molar ratio of o-vanillin (C₈H₈O₃) and L-cystine (C₆H₁₂N₂O₄S₂). Elemental analysis, IR and NMR spectroscopy were employed to characterize the composition and structure of OVCS. The analytical results show that the molecular formula of the Schiff base is Na₂(C₂₂H₂₂N₂O₈S₂). The thermokinetic properties of the action of OVCS on the growth metabolism of Schizosaccharomyces pombe were investigated by a TAM air isothermal calorimetry at 305.15 K. Some thermokinetic parameters of growth metabolism of S. pombe, such as the maximum heating power (P_max), growth rate constant (k), generation time (t_G), inhibition rate (I) and half inhibitory concentration (C_{I, 50}) were calculated. Experimental results indicate that the values of k, Q_total and P_max of S. pombe decrease, while the value of I, t_max and t_G of S. pombe increased with the increase of the concentrations of OVCS. The C_{I, 50} value of OVCS was found to be 35.99 mg/L (or 9.62×10^-2 mol/L). From this work, one can reach a conclusion that OVCS possesses inhibitory effect on the growth metabolism of S. pombe, and the greater the concentration, the stronger the inhibition.