大叶胡枝子根皮提取物抗炎镇痛作用研究

目的探讨大叶胡枝子根皮提取物灌胃给药的抗炎镇痛作用。方法采用二甲苯致小鼠耳肿胀、蛋清致大鼠足跖肿胀2种急性炎症模型以及大鼠棉球肉芽肿慢性炎症模型,研究大叶胡枝子根皮提取物灌胃给药对急慢性炎症的作用。采用热板法和醋酸扭体法致痛,研究大叶胡枝子根皮提取物灌胃给药的镇痛作用。结果与模型组相比,大叶胡枝子根皮水提物和醇提物高、中、低剂量组显著抑制二甲苯所致小鼠耳肿胀(P 0.01),明显抑制蛋清所致大鼠足跖肿胀(P 0.01),明显抑制大鼠棉球肉芽肿(P 0.01)。大叶胡枝子根皮水提物和醇提物高、中、低剂量组给药后明显提高热板所致小鼠的痛阈值,与模型组和自身给药前比较差异显著(P 0.05、0.01);大叶胡枝子根皮水提物和醇提物高、中、低剂量组明显减少醋酸所致小鼠扭体反应次数,与模型组比较差异显著(P 0.05、0.01)。大叶胡枝子根皮水提物的抗炎镇痛作用强于醇提物(P 0.05、0.01)。结论大叶胡枝子根皮提取物灌胃给药具有一定抗炎、镇痛作用,且其水提物强于醇提物。

ANTI-INFLAMMATORY AND ANALGESIC EFFECTS OF THE ROOT BARK EXTRACT OF LESPEDEZA DAVIDII

Objective: To investigate the anti-inflammatory and analgesic effects of the root bark extract of Lespedeza davidii by intragastric administration. Methods: The anti-inflammatory animal models were used in researching the effects of the extract on acute and chronic inflammation. The acute models were xylene-induced ear edema and egg white-induced paw edema, while the chronic one was granuloma induced by cotton pellet implantation. Meanwhile, the analgesic effects of that extract were observed by hot plate and acetic acid writhing test. Results: Compared with the model group, high, middle and low doses of the root bark water extract and alcohol extract of L. could significantly inhibit ear edema in mice (P<0.01) and the paw edema in rat (P<0.01). Also, the water and alcohol extract decreased the granuloma of rats (P<0.01). Moreover, the pain threshold with hot plate method was significantly increased (P<0.05, 0.01) compared with the model group and the data before the drugs administration of high, middle and low doses of the water extract and alcohol extract. And contrasted to the model group after extract administration, the writhing number was reduced (P<0.05, 0.01). However, there were substantial variations in the water extract groups and the same dose of alcohol extract in high, middle and low concentrations (P<0.05, 0.01). Conclusion: The root bark extract of L. possesses the anti-inflammatory and analgesic effects, and water extract were better than alcohol extracts.