基于化学计量学黄花白及和小白及HPLC指纹图谱分析

采用高效液相色谱（HPLC）法建立6批黄花白及和8批小白及指纹图谱并比较Militarine含量，利用相似度（SA）、聚类分析（HCA）和主成分分析（PCA）对黄花白及和小白及进行综合评价。结果显示HPLC指纹图谱共生成26个共有峰，利用对照品指认12号色谱峰为Militarine，且其含量在二者之间具显著性差异（P<0.01）；SA分析显示黄花白及和小白及相似度大于0.912，具较高的相似性；HCA和PCA将黄花白及和小白及分为两类，表明两者存在一定的差异性，分析结果相互验证。结果表明黄花白及和小白及化学组成类似，HPLC指纹图谱技术结合化学计量学方法适用于两者的区分和内在质量评价。

FINGERPRINT ANALYSIS OF BLETILLA OCHRACEA AND BLETILLA FORMOSANA BASED ON HPLC COMBINED WITH CHEMOMETRICS METHODS

High performance liquid chromatography (HPLC) fingerprints of 6 batches of Bletilla ochracea and 8 batches of B. formosana were established and the content of Militarine was compared. The quality assessment was achieved by using similarity analysis (SA), hierarchical clustering analysis (HCA) and principal component analysis (PCA). The results indicated that twenty-six common characteristic peaks were found in all tested samples, peak 12 was identified as Militarine with significant differences in content (P<0.01); Similarity values were all above 0.912, which indicated the tested samples has high similarity; All above results could validate each other and successfully group these samples into two categories, which showed the samples are different. The results showed that all tested samples have similar chemical composition, HPLC fingerprint technology combined with chemometrics methods could be used for differentiation and quality assessment on the B. ochracea and B. formosana samples.