治疗干预对大鼠结核性脑膜炎蛛网膜颗粒结构的影响

目的观察药物治疗干预措施对结核性脑膜炎大鼠蛛网膜颗粒结构的影响,为研究结核性脑膜炎继发脑积水形成机制和药物干预治疗提供理论依据。方法建立大鼠结核性脑膜炎模型,分为A、B、C、D组(实验组),实验A、B、C组均给予异烟肼及利福平抗结核治疗,其中B、C组分别另外经小脑延髓池注射尿激酶(B)、乌司他丁(C),D组为非治疗干预组,E组为健康空白对照组。第3周,处死大鼠,之前经小脑延髓池注入亚甲蓝定位蛛网膜颗粒,使用TUNEL法观察5组大鼠蛛网膜颗粒细胞凋亡情况差异。结果 A、B、C组蛛网膜颗粒中偶见细胞凋亡,非治疗干预D组蛛网膜颗粒中凋亡细胞增多,对照组未见凋亡。结论大鼠结核性膜炎后存在广泛的蛛网膜下腔炎症反应过程,使蛛网膜颗粒出现过度的细胞凋亡,治疗干预可能减少蛛网膜颗粒细胞凋亡。

EFFECTS OF INTERVENTION TREATMENT ON THE GRANULATIONS STRUCTURE OF RAT TUBERCULOUS MENINGITIS ARACHNOID

objective: To observe the effects of drug treatment interventions on the granulations structure of rat tuberculous meningitis arachnoid, to provide theoretical basis for investigating the formation mechanism of tuberculous meningitis secondary hydrocephalus and drug intervention treatment. Methods: The rat model of tuberculous meningitis was established, all rats were divided into group A, B, C, D as the experimental group, the experimental group A, B, C were treated with isoniazid and rifampicin for anti-tuberculosis treatment, in addition, group B and C respectively were injected with urokinase (B) and ulinastatin (C) through the transcerebellar medulla oblongata pool. Group D was set as a therapeutic intervention group, and group E as health blank control group. After 3 weeks, the rats were killed after being injected with methylene blue for positioning arachnoid granulations through the transcerebellar medulla oblongata pool, the differences of arachnoid granulations apoptosis of rats in 5 groups were observed by using TUNEL method. Results: Arachnoid granulations apoptosis in group A, B were occasionally observed, apoptosis cells in group D increased, and wasn''t found in the control group. Conclusions: Tuberculous meningitis in rats widely results in subarachnoid inflammation process, make the arachnoid granulations appear excessive apoptosis, treatment interventions could reduce the apoptosis of arachnoid granulations.