| 产γ-氨基丁酸乳酸菌的筛选 |
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| 引用本文： | 林谦,廖珊珊.产γ-氨基丁酸乳酸菌的筛选[J].玉林师范学院学报,2014(5):50-53. |
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| 作者姓名： | 林谦 廖珊珊 |
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| 作者单位： | 玉林师范学院生命科学与技术学院,广西玉林537000 |
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| 基金项目： | 广西自然科学基金（2011GXNSFB018046）; 广西高校特色专业及课程一体化建设项目（GXTSZY220,GXTSZY022） |
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| 摘 要： | 从多种酸菜水中分离筛选出乳酸菌,利用乳酸菌将谷氨酸转化为GABA的脱羧作用,通过混合酸碱指示剂颜色变化,直观判断谷氨酸脱羧酶（GAD）活性,进而筛选出产GABA乳酸菌菌株BL2,并对其进行初步鉴定;同时,采用纸层析法进行定性分析,确定菌株BL2可将谷氨酸钠转化生成GABA.
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| 关 键 词： | 乳酸菌 γ-氨基丁酸 筛选 |
Screening of Lactic Acid Bacteria for Synthesis of γ-Aminobutyric Acid |
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| LIN Qian,LIAO Shan-shan.Screening of Lactic Acid Bacteria for Synthesis of γ-Aminobutyric Acid[J].Journal of Yulin Teachers College,2014(5):50-53. |
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| Authors: | LIN Qian LIAO Shan-shan |
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| Institution: | (College of Life Science and Technology, Yulin Normal University, Yulin, Guangxi 537000) |
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| Abstract: | LAB Strains were isolated from pickled vegetable juice. Since glutamate acid decarboxylase of LAB can convert monosodium glutamate toγ-aminobutyric acid, color changes of transformation system supplemented with acid-base indicator facilitate screening of GABA-producing LAB. Strain BL2 was isolated and identified. Analysis of paper chromatography suggested the strain＇s ability to produce GABA. |
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| Keywords: | lactic acid bacteria γ-aminobutyric acid screening |
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