| PP纤维接枝聚丙烯酰胺/海藻酸钙蛋白质印迹水凝胶的制备 |
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| 引用本文： | 林贝贝,赵孔银,冯灵智,崔文葵,魏俊富.PP纤维接枝聚丙烯酰胺/海藻酸钙蛋白质印迹水凝胶的制备[J].中国科技论文在线,2014(3):275-278,282. |
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| 作者姓名： | 林贝贝 赵孔银 冯灵智 崔文葵 魏俊富 |
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| 作者单位： | [1]天津工业大学中空纤维膜材料与膜过程国家重点实验室培育基地,天津300387 [2]天津工业大学材料科学与工程学院,天津300387 |
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| 基金项目： | 高等学校博士学科点专项科研基金资助项目（20111201120004,20101201110001）;国家自然科学基金资助项目（51103102） |
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| 摘 要： | 以PP纤维为载体,牛血清白蛋白(BSA)为模板,丙烯酰胺为单体,N,N′-亚甲基双丙烯酰胺为交联剂,在海藻酸钠的存在下,采用紫外辐射引发,制备了PP纤维接枝BSA印迹聚丙烯酰胺/海藻酸钙水凝胶(PP-g-PAM/CA MIP)。研究了单体浓度、交联剂浓度和海藻酸钠浓度对BSA吸附量和印迹效率的影响,结果表明,单体质量分数为10%,交联剂质量分数为3%,海藻酸钠质量分数为0.5%时,PP接枝的印迹水凝胶对BSA有最大吸附量。
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| 关 键 词： | 聚丙烯无纺布 蛋白质 分子印迹 水凝胶 接枝 丙烯酰胺 |
Preparation of protein imprinted calcium alginate/polyacrylamide hydrogel membrane grafted on polypropylene fibers |
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| Lin Beibei,Zhao Kongyin,Feng Lingzhi,Cui Wenkui,Wei Junfu.Preparation of protein imprinted calcium alginate/polyacrylamide hydrogel membrane grafted on polypropylene fibers[J].Sciencepaper Online,2014(3):275-278,282. |
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| Authors: | Lin Beibei Zhao Kongyin Feng Lingzhi Cui Wenkui Wei Junfu |
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| Institution: | 1. State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China ; 2. School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China) |
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| Abstract: | Bovine serum albumin （BSA） imprinted polypropylene （PP） fiber-grafted polyacrylamide/calcium alginate hydrogel （PP-gPAM/CA MIP） has been prepared using non-woven PP fiber as matrix, with BSA as template molecule, N, N＇-methylenebisacrylamide as the cross-linker, and acrylamide （AM） as functional monomer, via UV radiatiomreduced polymerization in the presence of sodium alginate （SA）. Factors influencing the adsorption capacity of PP-g-PAM/CA MIP, such as monomer concen tration, cross linker concentration and sodium alginate concentration, were also investigated. The results showed that when the monomer mass fraction was 10 %, crosslinking agent mass fraction was 3 %, alginate sodium mass fraction was 0.5 %, the PP-g- PAM/CA MIP had a maximum adsorption capacity for BSA. |
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| Keywords: | polypropylene non-woven protein molecule imprinting hydrogel grafting acrylamide |
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