Th17细胞相关细胞因子与急性移植物抗宿主病关系的探讨

目的探讨异基因造血干细胞移植治疗血液系统疾病后,血清中Th17细胞相关的细胞因子与急性移植物抗宿主病的关系。方法 78例血液病患者进行异基因造血干细胞移植,对移植后发生急性移植物抗宿主病患者(n=12),未发生急性移植物抗宿主病患者(n=14),及健康对照组(n=10),以及发生急性移植抗宿主病患者(n=10)在有效治疗前后,采用酶联免疫吸附法检测血清中细胞因子IL-17、IL-23、IL-21、IFN-γ、TGF-β1、IL-22浓度。结果 1 aGVHD者(n=12)血清中Th17细胞相关因子浓度(pg/mL)较正常对照组(n=10)和无aGVHD组(n=14)显著增高,差异有统计学意义(P<0.05);而TGF-β1(1 156.61±458.04)显著低于正常对照组(2 978.34±1 647.71)和无aGVHD组(2 541.62±1 805.16),差异有统计学意义(P<0.05)。IL-22:未发生aGVHD组高于健康对照组(22.14±2.49,19.18±1.24),差异有统计学意义(P<0.05);除IL-21、IL-22和IL-23浓度高于健康对照组外(P<0.05),其余的细胞因子健康对照组和无急性aGVHD组差异无统计学意义(P>0.05),aGVHD组血清IL-22浓度与无aGVHD和健康对照组比较,差异无统计学意义(P>0.05)。210例有效治疗者,治疗后均有降低,治疗前后差异有统计学意义(P<0.05),而IL-22在有效治疗前后差异无统计学意义(P>0.05)。结论 Th17细胞相关的细胞因子与(IL-17、IL-23、IL-21、IFN-γ、TGF-β1)与aGVHD的发生发展和疗效有关,而与IL-22无关。

Relationship of Cytokines Associated with Th17 Cells and Acute Graft-versus-host Disease

Objective To explore the relationship of cytokones associated with Th17 cells in serum and acute graft-versus-host disease from patients with hematologic diseases who were undergone al-logeneic hematopoietic stem cell transplantation. Methods In this study,78 patients with haemato-logical diseases were undergone allogeneic hematopoietic stem cell transplantation. Th17 cell-associ-ated cytokines in serum were examined by enzyme linked immunosorbent assay（ELISA）from pa-tients with acute graft-versus-host disease（aGVHD）（n=12）,without aGVHD（n=14）,and patients with aGVHD who were effectively treated（n=10）. Healthy volunteers （n=10） were severed as control. Results ①The serum cytokine levels（pg/mL）of patients with aGVHD（n=12）were signifi-cantly higher compared with healthy control group（n=10）and non-aGVHD group（n=14）（P＆lt;0. 05）. On contrary,The TGF-β1 level（pg/mL）（1 156. 61 ＆#177; 458. 04） was significantly lower than the healthy control group（2 978. 34 ＆#177; 1 647. 71）and non-aGVHD group（2 541. 62 ＆#177; 1 805. 16）（P＆lt;0. 05）. IL-22：non-aGVHD group was significantly higher than healthy control group（22. 14＆#177;2. 49, 19. 18＆#177;1. 24）（P＆lt;0. 05）;IL-21,IL-22,IL-23 were higher than healthy control group（P＆lt;0. 05）, there was no statistical difference about other cytokines between healthy control group and non-aGVHD group（P＆gt;0. 05）.②After treatment,the cytokine levels above in 10 cases of effective treatment decreased to considerable extent ,and there was significant difference between before and after effective treatment（P ＆lt;0. 05）,but the IL-22 showed no significant difference between before and after effective treatment （P＆gt;0. 05）. Conclusion Th17 cell-associated cytokines（IL-17, IL-23,IL-21,IFN-γ,TGF-β1）in serum are assocoated with pathogenesis,development and treat-ment efficacy of aGVHD,whereas acute GVHD is no relationship with IL-22 cencentration in serum.