p63在胃癌组织中的表达

目的:探讨抑癌基因p53蛋白家族成员p63蛋白在胃癌及癌旁组织中的表达情况.方法:回顾性分析41例胃癌及37例癌旁组织,利用组织微阵列技术,构建88点组织阵列,并采用免疫组织化学技术S-P法检测该阵列中p63蛋白在胃癌及其癌旁组织中表达情况.结果:①p63蛋白定位于细胞核,呈弥漫分布的棕黄色颗粒;②胃癌组织中,p63蛋白表达于核大间变细胞中;p63蛋白在高分化型胃癌中阳性表达率为5.9%,在低分化型胃癌中的阳性表达率为41.7%,两者比较差异有统计学意义(P<0.05);③p63蛋白在胃癌癌旁组织中无表达,在胃癌中的阳性表达率为24.4%,两者比较差异有统计学意义(P<0.05).结论:①p63蛋白的高表达可能参与了胃癌的发生;②p63与胃癌的分化程度有关,并可能参与了胃癌细胞的分化.

Expression of p63 Protein in Gastric Cancer

Objective：To discuss the expression of p63 protein of p53 gene family member in gastric cancer and its significance. Method：The tissue microarrary was made from 41 cases of gastric cancer and 37 cases of normal tissues nearby the gastric cancer,and the expression of p63 was detected by immunohistochemical S-P methods. Results：① p63 protein shows as claybank particles which were distributed in nucleus.② The expression of p63 protein were in anaplastic cell. The positive expression rates of p63 protein in well-differentiated gastric cancer and low-differentiated gastric cancer were 5.9% and 41.7%, and the comparison has statistical significance（p0.05）.③ The expression of p63 protein in normal tissues nearby the gastric cancer were negative,together with the positive expression rates of p63 protein in gastric tissue were 24.4 %,and the comparison has statistical significance（p 0.05）.Conclusions：①The hyper-expression of p63 protein maybe join the development of gastric cancer. ② p63 protein concerns with the differentiation grade of gastric cancer and may be involved in cell differentiation of gastric cancer .