| 聚乙烯基吡咯烷酮对质粒DNA降解效应的研究 |
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| 引用本文： | 林燕,姚晶,李佳,蒋琳,李正,梁勇.聚乙烯基吡咯烷酮对质粒DNA降解效应的研究[J].江汉大学学报(自然科学版),2011,39(4):82-86. |
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| 作者姓名： | 林燕 姚晶 李佳 蒋琳 李正 梁勇 |
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| 作者单位： | 江汉大学医学院,湖北武汉,430056 |
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| 基金项目： | 国家自然科学基金(20907017,20890112) |
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| 摘 要： | 为评估聚乙烯基吡咯烷酮（Polyvinylpyrrolidone,PVP）的生物可利用性,研究了不同相对分子质量PVP对闭合环状双链质粒DNA pBR322的降解效应.实验结果表明：在常温、非光催化条件下,相对分子质量小的PVP（PVP8000）可引起质粒DNA构象的改变,并且这种效应与反应温度、反应时间及PVP的相对...
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| 关 键 词： | PVP DNA构象 DNA剪切 质粒DNA |
Study on Degradation Effect of Polyvinylpyrrolidone on Plasmid DNA |
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| LIN Yan,YAO Jing,LI Jia,JIANG Lin,LI Zheng,LIANG Yong.Study on Degradation Effect of Polyvinylpyrrolidone on Plasmid DNA[J].Journal of Jianghan University:Natural Sciences,2011,39(4):82-86. |
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| Authors: | LIN Yan YAO Jing LI Jia JIANG Lin LI Zheng LIANG Yong |
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| Institution: | LIN Yan,YAO Jing,LI Jia,JIANG Lin,LI Zheng,LIANG Yong(School of Medicine,Jianghan University,Wuhan 430056,Hubei,China) |
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| Abstract: | To evaluate the bioavailability of Polyvinylpyrrolidone(PVP),compared the DNA degradation effect of PVP with different molecular weight on closed circular double-stranded plas-mid pBR322.The results revealed that the lower molecular weight PVP(PVP 8 000) could sig-nificantly degrade the plasmid DNA pBR322 under normal temperature without photoactivation,which was correlated to reaction temperature,reaction time,molecular weight and concentration of PVP.In addition,when different concentrations of formamide ... |
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| Keywords: | PVP DNA conformation DNA degradation plasmid DNA |
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