| 热处理对HeLa细胞微管影响的研究 |
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| 引用本文： | 刘宇炜,鲁雄兵,赵林.热处理对HeLa细胞微管影响的研究[J].江汉大学学报(自然科学版),2005,33(1):80-82. |
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| 作者姓名： | 刘宇炜 鲁雄兵 赵林 |
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| 作者单位： | 江汉大学,医学与生命科学学院,湖北,武汉,430056;江汉大学,医学与生命科学学院,湖北,武汉,430056;江汉大学,医学与生命科学学院,湖北,武汉,430056 |
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| 摘 要： | 目的:用HeLa细胞作模型,研究热处理对肿瘤细胞微管的影响.方法:用不同温度(37°C、、40°C43°C和45°C)经不同时间(1h和2h)水浴处理培养的HeLa细胞后,分别即时用SABC法显示其微管.结果:与37°C相比,40°C处理后的HeLa细胞微管变化不大,43°C处理后,微管开始解聚,并随着作用时间的延长而加剧,45°C处理2h,微管组织中心消失.结论:高温能引起HeLa细胞微管呈现渐进式的解聚变化,微管组织中心具有较强的保护作用.
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| 关 键 词： | 热处理 HeLa细胞 微管 |
| 文章编号： | 1673-0143(2005)01-0080-03 |
| 修稿时间： | 2003年8月27日 |
Researches of Effects on Microtubules of HeLa Cells After Heat Treatment |
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| LIU Yu-wei,LU Xiong-bing,ZHAO Ling.Researches of Effects on Microtubules of HeLa Cells After Heat Treatment[J].Journal of Jianghan University:Natural Sciences,2005,33(1):80-82. |
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| Authors: | LIU Yu-wei LU Xiong-bing ZHAO Ling |
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| Abstract: | Objective: Using HeLa cells as a model, researched the effects of its microtubules after heat treatment. Methods: Cultured HeLa cells were incubated in four incubators at different temperatures (37℃, 40℃, 43℃ and 45℃) for 1h or 2h, using SABC method to show its microtubules immediately.Results: To compare with groupsof 37℃, the distribution of microtubules had littlechanges; at 43℃, depolymerization of microtubules happened, aggravated following the elongation of time; at 45℃ for 2h, the microtubule organizing center (MTOC) disappeared. Conclusion: Hyperthermia can lead to depolymerization of microtubules of HeLa cells gradually. MTOC may have strong protection. |
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| Keywords: | heat treatment HeLa cells microtubules |
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