IL-6对大鼠脑缺血再灌注后海马及纹状体NOS和Fos表达的影响

目的 :研究IL 6对大鼠脑缺血再灌注 (CIRF)后海马及纹状体的NOS和Fos阳性神经元表达的作用。方法 :采用大脑中动脉栓塞法 (MCAO)对 2 4只Wistar大鼠左侧大脑中动脉构成CIRF模型的基础上分两组 :即单纯CIRF对照组 (n =8) ;经同侧脑室事先注入IL 6的实验组 (n =16)。分别进行NADPH脱氢酶反应显示NOS和免疫组织化学染色显示Fos表达。观察了CIRF后 1、2、4、6小时海马及纹状体处NOS和Fos阳性神经元数量的变化 ,以及IL 6对其变化的影响。结果 :大鼠CIRF后纹状体缺血中心区缺少NOS和Fos的表达 ,而纹状体周围区NOS和Fos阳性神经元明显增加 ;海马各部NOS和Fos阳性神经元均明显增加 ,其中CA1区增加最显著。但预先经侧脑室注射IL 6的实验组中 ,大鼠海马及纹状体NOS和Fos阳性神经元明显低于对照组。IL 6明显抑制了NOS及Fos表达。结论 :提示IL 6可能参与脑缺血性神经元损伤的调控作用

Effects of Cytokines(Interleukin-6) on NOS and Fos expression of Hippocampal and Striatal Neurons during Focal Cerebral Ischemia Reperfusion in Rats

Objective: In order to study the changes of NOS and Fos expression of Hippocampal and Striatal neurons during focal cerebral ischemia reperfusion in rats. Methods: 24 Wistar rats were divided into two groups based on the model of middle cerebral artery(MCA) focal cerebral ischemia reperfusion, that is, a control group (simple ischemia reperfusion, n =8) and a experimental group (injected IL-6 into the same lateral ventricle before ischemia, n =16).By the staining of NADPH histochemistry and Fos immunohistochemisty, respectively, the changes of NOS and Fos expression of Hippocalmpal and Striate neurons were observed during ischemia reperfusion 1h, 2h, 4h and 6h.Results: The results showed that the expression of NOS and Fos positive neurons in the core of striate ischemia area were deficient. Whereas in the periphery of striate area, the expression increased markedly. The amount of NOS and Fos positive neurons in Hippocampus increased obviously, with the most significantly increasing in the CA1 area. Furthermore, IL-6 inhibited the expression of NOS and Fos significantly.Conclusion: It is indicated that IL-6 might participate in the modulation of cerebral ischemia reperfusion.