A novel fluorescent label based on biological fluorescent nanoparticles and its application in cell recognition |
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Authors: | Xiaoxiao He Kemin Wang Weihong Tan Dan Xiao Jun Li Xiaohai Yang |
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Institution: | (1) College of Chemistry & Chemical Engineering, Hunan University, 410082 Changsha, China |
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Abstract: | Uniform-sized fluorescent nanoparticles have been prepared by employing silica as the shell and a highly luminescent dye complex
of ruthenium ion and bipyridyl, tris(2,2’-bipyridyl) dichlororuthenium(II) hexahydrate as the core of the nanoparticles. A
novel fluorescent label method is proposed, which is based on the biological fluorescent nanoparticles on the foundation of
nanotechnology, biotechnology and fluorescent label technology. In comparison with the conventional fluorophores as fluorescent
labels such as fluorescein isothiocyanate (FITC) label, this new label shows more superiority in photochemical stability,
detection sensitivity and application scope for the biomedicine research. SmIgG+ B lymphocytes isolated from the circulating blood of human beings can be easily recognized by using this new fluorescent
label. |
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Keywords: | biological fluorescent nanoparticles fluorescent label cell recognition |
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