| Measurements of leucocyte membrane elasticity based on the optical tweezers |
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| 作者姓名： | GUOHonglian CAOQinhong RENDongtao LIUGuoqing DUANJianfa LIZhaolin ZHANGDaozhong HANXuehai |
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| 作者单位： | [1]OpticalPhysicsLaboratory,InstituteofPhysicsandCenterforCondensedMatterPhysics,ChineseAcademyofSciences,Beijing100080,China [2]StateKeyLoboratoryofPlantPhysiologyandBiochemistry,CollegeofBiologySciences,ChinaAgriculturalUniversity,Beijing100094,China [3]StateKeyLaboratoryofBiomacromolecles,InstitueofBiophysics,ChineseAcademyofSciences,Beijing100101,China [4]StateKeyLaboratoryofBiomacromolecles,InstituteofBiophysics,ChineseAcademyofSciences,Beijing100101,China |
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| 基金项目： | the National Natural Science Foundation of China (Grant Nos. 1989380 and 10174097) |
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| 摘 要： | A 1-μm in diameter polystyrene bead coated with lectin is trapped by optical tweezers whics is formed by a focused laser beam (740nm).A leucocyte adhered to the bottom of sample cell is chosen to close to the trapped bead.When their stable combination is confirmed,the leucocyte is displaced by moving the sample stage.A“tether”is then formed between the trapped bead and the membrane.The force acting on the tether is measured by a detector equipped on the optical tweezers system.The deformation of the membrane is diverse in different contact conditions.When the contact area is small,the tether is very thin and the force on it is 7.4pN(10^-12N),while the tether is much thicker under a larger contact area and its corresponding force is about 14pN.It is presumed that the latter is related to the deformation of cytoskeleton.
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| 关 键 词： | 白细胞 细胞膜 弹力测量 光学镊子系统 细胞骨架 血液细胞 |
| 收稿时间： | 2002-10-15 |
Measurements of leucocyte membrane elasticity based on the optical tweezers |
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| GUOHonglian CAOQinhong RENDongtao LIUGuoqing DUANJianfa LIZhaolin ZHANGDaozhong HANXuehai.Measurements of leucocyte membrane elasticity based on the optical tweezers[J].Chinese Science Bulletin,2003,48(5):503-508. |
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| Authors: | GUO Honglian CAO Qinhong REN Dongtao LIU Guoqing DUAN Jianfa Li Zhaolin ZHANG Daozhong HAN Xuehai |
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| Institution: | GUO Honglian1*, CAO Qinhong3*, REN Dongtao3, LIU Guoqing3, DUAN Jianfa2, LI Zhaolin1, ZHANG Daozhong1 & HAN Xuehai2 1. Optical Physics Laboratory, Institute of Physics and Center for Con-densed Matter Physics, Chinese Academy of Sciences, Beijing 100080, China; 2. State Key Laboratory of Biomacromolecles, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 3. State Key Loboratory of Plant Physiology and Biochemistry, College of Biology Sciences, China |
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| Abstract: | A 1-mm in diameter polystyrene bead coated with lectin is trapped by optical tweezers which is formed by a focused laser beam (740 nm). A leucocyte adhered to the bottom of sample cell is chosen to close to the trapped bead. When their stable combination is confirmed, the leucocyte is displaced by moving the sample stage. A tether is then formed between the trapped bead and the membrane. The force acting on the tether is measured by a detector equipped on the optical tweezers system. The deformation of the membrane is diverse in different contact conditions. When the contact area is small, the tether is very thin and the force on it is 7.4 pN (10-12 N), while the tether is much thicker un-der a larger contact area and its corresponding force is about 14 pN. It is presumed that the latter is related to the defor-mation of cytoskeleton. |
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| Keywords: | optical tweezers elasticity of membrane tension of membrane cytoskeleton |
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