Cytoskeleton-associated protein tyrosine phosphorylation involved in induction of differentiation in mouse melanoma cells |
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Authors: | Chunhong Yan Rui Han |
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Institution: | (1) Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 100050 Beijing, China |
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Abstract: | The malignancy of a cancer is due partly to its poor differentiation. Genistein, a protein tyrosine kinase inhibitor, is found
to induce the highly malignant B16-BL6 mouse melanoma cells to differentiate to mature phenotypes. When Triton X-100 insoluble
fraction of the differentiated cells is prepared and analyzed, tyrosine phosphorylation levels of three cytoskeleton-associated
proteins (65, 60 and 53 ku respectively) are found to decrease dramatically. But no any change is found when phosphotyrosine
contents of the cytosol fraction or the total cellular protein preparations are evaluated. It is concluded that cytoskeleton-associated
protein tyrosine phosphorylation may be involved in the control of differentiation of cancer cells. The decrease of phosphotyrosine
contents of cytoskeleton-associated proteins may be one of the important mechanisms underlying the differentiation induction
of cancer cells by anticancer agents. |
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Keywords: | cytoskeleton-associated protein protein tyrosine phosphorylation cancer cell differentiation protein tyrosine kinase inhibitor |
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