Cloning oflea cDNA fragment of carrot (Daucus carota L.) and analysis of its expression features |
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Authors: | Mei Qi Fan Chen Meijuan Huang Naihu Wu |
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Institution: | (1) Institute of Developmental Biology, Chinese Academy of Sciences, 100080 Beijing, China;(2) College of Life Sciences, Peking University, 100871 Beijing, China |
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Abstract: | Addition of concentrated sucrose to MS culture arrests the development of carrot somatic embryo at the stage of cotyledon
embryo and, with the sucrose concentration restored to normal level, the embryo thus arrested is reactivated into post-embryonic
development. Using the method of RT-PCR, the cDNA fragment of a new member of the Dc3 family of lea has been obtained from
carrot somatic embryo under regulated state. As revealed by Northern blotting, strong expression has been observed in carrot
somatic embryo under regulated state but the expression was much reduced 12 h after deregulation, and nearly disappeared 24
h after. Based on this finding as well as results of related studies, it is surmised that changing the sucrose concentration
in culture enabled carrot somatic embryo under suspension culture to undergo a specific course of development which is comparable
to the dormancy-germination process of seeds. |
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Keywords: | carrot somatic embryo regulated system lea expression control |
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