Induction of human β-defensin-2 in airway epithelial cells by Streptococcus pneumoniae: involvement of IP3-dependent intracellular calcium release and NF-κB |
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作者姓名: | Wei Liao Yan Wang Fang Zhang Congmin Zhao |
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作者单位: | Department of Pediatrics,Southwest Hospital,The Third Military Medical University;Department of Pediatrics Xinqiao Hospital,The Third Military Medical University;Institute of Respiratory Diseases,Xinqiao Hospital,The Third Military Medical University |
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基金项目: | supported by the National Natural Science Foundation of China(81170034) |
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摘 要: | Human β-definsin-2 (hBD-2) is mainly induced by bacterial factors and pro-inflammation mediators in epithelial cells. As the major cause of community-acquired pneumonia, whether Streptococcus pneumoniae (S. pneu-moniae) stimulation induces hBD-2 expression in airway epithelial cells is elusive. In this study, we found that S. pneumoniae stimulation induced hBD-2 expression in a time-and concentration-dependent manner in primary human airway epithelial cells. To further reveal the mechanism of S. pneumoniae inducing hBD-2, we found that S. pneumoniae stimulation activated NF-κB signaling pathway. Specific NF-κB inhibitor, PDTC, could reverse the induction of hBD-2 by S. pneumoniae. We also found that cellular inner Ca^2+ signaling is involved in the S. pneumoniae-induced hBD-2. Taken together, our find-ings indicated that S. pneumoniae can stimulate the expression of hBD-2 in airway epithelial cells and NF-κB and inositol triphosphate-dependent intracellular calcium release is involved in this induction.
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关 键 词: | 肺炎链球菌 上皮细胞 钙释放 细胞内 诱导 气道 人类 防御素 |
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