Semi-crosslinked polyacrylamides as high-resolution and dynamic self-coating sieving matrices for protein capillary electrophoresis |
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Authors: | Jin Zhou JianDong Xu Yao Xie Feng Qu YuLin Deng LiNa Geng |
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Institution: | (1) School of Life Science and Technology, Beijing Institute of Technology, Beijing, 100081, China |
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Abstract: | This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked polyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes. |
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Keywords: | capillary sieving electrophoresis semi-crosslinked polyacrylamide protein separation |
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