Transgenic tobacco plants expressing synthetic Cry1Ac and Cry1Ie genes are more toxic to cotton bollworm than those containing one gene |
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Authors: | Yun Lian ZhiWei Jia KangLai He YunJun Liu FuPing Song BaoMin Wang GuoYing Wang |
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Institution: | (1) State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100094, China;(2) State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agriculture Sciences, Beijing, 100094, China;(3) Institute of Crop Sciences, Chinese Academy of Agricalture Sciences, Beijing, 100081, China |
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Abstract: | Transgenic tobacco plants carrying Cry1Ac, Cry1Ie or both genes were obtained. In the leaves of transgenic plants carrying both genes, the contents of Cry1Ac and Cry1Ie proteins
were 0.173% and 0.131% of the total proteins, respectively. Cry1Ac protein content was 0.182% and Cry1Ie protein content was
0.124% of the total proteins in the leaves of transgenic plants containing only one Bt gene. Fresh leaves of transgenic tobacco
and wild-type plants were used for the insect bioassay against wild-type and Cry1Ac-resistant cotton bollworm (Helicoverpa armigera). The bioassay results showed that transgenic plants carrying both genes were significantly more toxic to wild-type and Cry1Ac-resistant
cotton bollworm than those carrying Cry1Ac or Cry1Ie alone. This study indicates that the higher toxicity of transgenic tobacco plants carrying both genes is caused by the cooperative
function of both Bt proteins, thus providing a potential way to delay the development of insect resistance to transgenic crops.
Supported by the National Plant Transformation and Industrialization Program of China (Grant No. JY03-A-13) |
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Keywords: | Cry1Ac Cry1Ie transgenic tobacco toxic Bt-resistant cotton bollworm |
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