Expression analysis ofgdcsP promoter from C3-C4 intermediate plantFlaveria anomala in transgenic rice |
| |
Authors: | Shuai Chen Nan Qu Shouyun Cao Bauwe Hermann Shouyi Chen Wenzhong Tian Chengcai Chu |
| |
Institution: | (1) Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China;(2) Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany;(3) Rostock University, 18055 Rostock, Germany |
| |
Abstract: | ThegdcsP promoter isolated from C3-C4 intermediate plantFlaveria anomala was fused to the β-glucuronidase (GUS) gene. The chimeric gene was inserted into the binary vector pBin19 and introduced
into the rice (Oryza sativa L.) cv. 8706 byAgrobacteriummediated gene transfer. GUS activity can be detected in leaf, leaf sheath, stem and root tissues via fluorometric GUS assay. However,
no GUS activity was found in mature endosperm. Histochemical localization revealed that GUS expression was exclusively restricted
to vascular tissues in transgenic plants. This promoter also showed spatial-temporal expression patterns that GUS expression
declined significantly with the maturity of plants. These expression patterns make thegdcsP promoter extremely valuable in the applied biotechnology that needs target gene expression restricted to vascular tissues. |
| |
Keywords: | rice (Oryza sativa L ) glycine decarboxylase P-protein subunit vascular tissue |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |