| Development of the CAS-IBB single-particle microbeam for localized irradiation of living cells |
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| 作者姓名： | WANGXufei WANGXiaohua CHENLianyun HUZhiwen LIJun WUYu CHENBin HUSuhua ZHANGJun XUMingliang WULijun WANGShaohu FENGHuiyun ZHANFuru PENGShixiang HUChundong ZHANGShuqing CHENGJianjun SHIZhongtao YUANHang YUANHaitao YUZengliang |
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| 作者单位： | [1]Thenamesoftheco-authorsareorderedaccordingtothenumberofstrokesoftheirsurnamesinChinesecharacters [2]KeyLaboratoryofIonBeamBioengineering,InstituteofPlasmaPhysics,ChineseAcademyofSciences,KeyLaboratoryofIonBeamBioengineeringinAnhuiProvince,Hefei230031,China |
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| 摘 要： | A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydrogen ions, produced by a van de Graaff accelerator, in an energy range of 2.0-3.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is collimated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation protocols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results.
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| 关 键 词： | CAS-IBB 活细胞 微光束装置 生物工程学 单颗粒照射 粒子检测精度 目标定位精度 |
| 收稿时间： | 2004-04-22 |
| 修稿时间： | 2004-06-23 |
Development of the CAS-LIBB single-particle microbeam for localized irradiation of living cells |
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| WANGXufei WANGXiaohua CHENLianyun HUZhiwen LIJun WUYu CHENBin HUSuhua ZHANGJun XUMingliang WULijun WANGShaohu FENGHuiyun ZHANFuru PENGShixiang HUChundong ZHANGShuqing CHENGJianjun SHIZhongtao YUANHang YUANHaitao YUZengliang.Development of the CAS-IBB single-particle microbeam for localized irradiation of living cells[J].Chinese Science Bulletin,2004,49(17):1806-1811. |
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| Authors: | Xufei Wang Xiaohua Wang Lianyun Chen Zhiwen Hu Jun Li Yu Wu Bin Chen Suhua Hu Jun Zhang Mingliang Xu Lijun Wu Shaohu Wang Huiyun Feng Furu Zhan Shixiang Peng Chundong Hu Shuqing Zhang Jianjun Cheng Zhongtao Shi Hang Yuan Haitao Yuan Zengliang Yu |
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| Institution: | WANG Xufei*,WANG Xiaohua*,CHEN Lianyun*,HU Zhiwen*,LI Jun,WU Yu,CHEN Bin,HU Suhua,ZHANG Jun,XU Mingliang,WU Lijun,WANG Shaohu,FENG Huiyun,ZHAN Furu,PENG Shixiang,HU Chundong,ZHANG Shuqing,CHENG Jianjun,SHI Zhongtao,YUAN Hang,YUAN Haitao & YU Zengliang Key Laboratory of Ion Beam Bioengineering,Institute of Plasma Physics,Chinese Academy of Sciences,Key Laboratory of Ion Beam Bioengi- neering in Anhui Province,Hefei 230031,China |
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| Abstract: | A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydrogen ions, produced by a van de Graaff accelerator, in an energy range of 2.0–3.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is collimated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation protocols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results. |
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| Keywords: | microbeam facility single-particle irradiation particle detection precision target location accuracy living cells |
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