Generation of transgenic wheat resistant to wheat yellow mosaic virus and identification of gene silence induced by virus infection |
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Authors: | Jin Dong Zhentian He Chenggui Han Xiulan Chen Lingdi Zhang Weihua Liu Yuepeng Han Jinrong Wang Yafeng Zhai Jialin Yu Yi Liu Yueyan Xiao |
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Institution: | (1) State Key Laboratory for Agrobiotechnology, China Agricultural University, 100094 Beijing, China;(2) Agricultural Institute of Lixiahe District, 225207 Yangzhou, China |
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Abstract: | The plasmid containing the promoter Act1, the coat protein (cp) gene of wheat yellow mosaic virus (WYMV) and the selectable bar gene, was delivered via particle bombardment, directly into immature embryos of a wheat cultivars. PCR and PCR-RFLP were employed to screen the existence of the cp gene in T0 and T1 generations. Seeds from the positive T1 plants were sowed in fields heavily contaminated with WYMV to detect their resistance. In field trial of virus infection, one of the transgenic wheat lines, P8-T2, exhibited highly disease-resistance. Western blot and RT-PCR analysis showed that the expression level of cp gene in the resistant transgenic line was reduced greatly compared to those susceptible to WYMV infection. This provided evidence to presume that the resistance obtained by the transgenic wheat line was stimulated by the mechanism of the virus induced gene silencing. |
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Keywords: | wheat yellow mosaic virus coat protein gene transgenic wheat resistance gene silencing |
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