JWA protein binds to α-tubulin in PC12 cells |
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作者姓名: | CHENHairong LIAiqun LIAiping ZHOUJianwei |
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作者单位: | JiangsuProvincialKeyLaboratoriesofHumanFunctionalGenomicsandofAppliedToxicology,SchoolofPublicHealth.NanjingMedicalUniversity,Nanjing210029,China |
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摘 要: | Our previous study elucidated that JWA protein was a newly identified microtubule-associated protein (MAP), which combined to and co-localized with α-tubulin. In the present study, we designed a series of experiments to explore if any interactions between JWA protein and α-tubulin existed and how JWA protein would functionally link to α-tubulin, especially in cell mitosis. Results of coimmnnoprecipitation, gene transfection and immnnoflnores-cence microscopy from PC12 and HEK293 cells provided strong evidence for a linkage between JWA protein and α-tubulin. Our data showed that JWA protein bound to α-tubulin stably no matter whether α-tubulin was polymerized or not. In addition, by using antisense oligonncleotides, cell cycle blocking agents and hypothermia disposal techniques, we also found the interaction between JWA protein and α-tubulin. The further analysis using flow cytometry and confocal microscopy showed that both proteins co-existed in PC12 cells and were independent on the cell cycle. In conclusion, JWA protein is a newly identified microtnbnle-associated protein, binds to α-tubulin, and probably plays an important role in regulation of microtnbnlar stability.
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关 键 词: | α-微管蛋白 JWA蛋白质 有丝分裂 基因转染 |
收稿时间: | 30 July 2003 |
修稿时间: | 2004-01-08 |
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