| Fluorescence study on the interactions of PAMAM dendrimers and their derivatives with bovine serum albumin |
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| 作者姓名： | WANG Yanming SONG Yu KONG Deling YU Yaoting |
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| 作者单位： | Bioactive Material Research Laboratory, Nankai University, Tianjin 300071, China |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China （Grant No. 30370405）. |
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| 摘 要： | The interactions of amino-terminated, and ethylenediamine core poly（amidoamine） （PAMAM） dendrimers and their derivatives with bovine serum albumin （BSA） were investigated by fluorescence spectroscopy. Experimental results showed that the fluorescence intensity of BSA decreased after the addition of different modified dendrimers, and the extent of the fluorescence quenching caused by various modified dendrimers strongly depends upon the different functional groups on their surfaces. We also investigated the influence of pH and ionic strength on the interaction between various modified dendrimers and BSA. Circular dichroism （CD） spectroscopic measurements showed that the content of α-helix structure of BSA decreased after the addition of different modified dendrimers, which indicated that dendrimers induced changes in the secondary structure of BSA.
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| 关 键 词： | PAMAM 高分子化合物 化学结构 荧光性 分色性 |
| 收稿时间： | 2005-03-23 |
| 修稿时间： | 2005-03-232005-04-09 |
Fluorescence study on the interactions of PAMAM dendrimers and their derivatives with bovine serum albumin |
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| WANG Yanming SONG Yu KONG Deling YU Yaoting.Fluorescence study on the interactions of PAMAM dendrimers and their derivatives with bovine serum albumin[J].Chinese Science Bulletin,2005,50(21):2436-2441. |
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| Authors: | Yanming Wang Yu Song Deling Kong Yaoting Yu |
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| Institution: | WANG Yanming, SONG Yu, KONG Deling & YU Yaoting Bioactive Material Research Laboratory, Nankai University, Tianjin 300071, China |
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| Abstract: | The interactions of amino-terminated, and ethylenediamine core poly(amidoamine) (PAMAM) dendrimers and their derivatives with
bovine serum albumin (BSA) were investigated by fluorescence spectroscopy. Experimental results showed that the fluorescence
intensity of BSA decreased after the addition of different modified dendrimers, and the extent of the fluorescence quenching
caused by various modified dendrimers strongly depends upon the different functional groups on their surfaces. We also investigated
the influence of pH and ionic strength on the interaction between various modified dendrimers and BSA. Circular dichroism
(CD) spectroscopic measurements showed that the content of α-helix structure of BSA decreased after the addition of different
modified dendrimers, which indicated that dendrimers induced changes in the secondary structure of BSA. |
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| Keywords: | poly(amidoamine) dendrimers bovine serum albumin fluorescence quenching circular dichroism DOI: 10 1360/982005-445 |
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