Salt tolerance conferred by over-expression ofOsNHX1 gene in Poplar 84K |
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Authors: | Shuyao Wang Qijun Chen Wenlong Wang Xuechen Wang Mengzhu Lu |
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Institution: | (1) Department of Life Science, Hunan University of Arts and Science, 415000 Changde, China;(2) State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100094 Beijing, China;(3) Research Institute of Forestry, Chinese Academy of Forestry, l00091 Beijing, China |
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Abstract: | OsNHX1 gene (Na+/H+ antiporter gene ofOryza sativa L.) was introduced into Poplar 84K withAgrobacterium tumefaciens- mediated transformation. PCR, Southern and Northern blot analysis showed thatOsNHX1 gene was incorporated successfully into the genome of Poplar 84K and expressed in these transgenic plants. Salt tolerance
test showed that three lines of transgenic plants grew normally in the presence of 200 mmol/L NaCl, while the Na+ content in the leaves of the transgenic plants grown at 200 mmol/L NaCl was significantly higher than that in plants grown
at 0 mmol/L NaCl. The osmotic potential in the transgenic plants with high salinity treatment was lower than that of control
plants. Our results demonstrate the potential use of these transgenic plants for agricultural use in saline soils. |
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Keywords: | Poplar 84K OsNHX1 transformation salt tolerance |
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