Assemble four-arm DNA junctions into nanoweb

DNA is of structural polymorphism, which is useful in nanoarchitecture; especially, four-arm DNA junctions can be used to assemble nanowebs. The static four-arm DNA junctions were designed and synthesized. One-arm DNA and two-arm DNA came out simultaneously with the four-arm DNA junction’s formation. A new method, termed the two-step method, was proposed and the productivity of four-arm DNA junctions was increased. A nanoweb was assembled successfully, but it showed irregularity itself. It was not the same as we expected. We consider that it is a result from the flexibility of four-arm DNA junction.